OV-6 Antibody (OV-6) [Unconjugated]

OV-6 was detected in immersion fixed BG01V human embryonic stem cells differentiated into hepatocytes using Mouse Anti-Human/Rat OV-6 Monoclonal Antibody (Catalog # MAB2020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu, Rt
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: OV-6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

OV-6 Antibody (OV-6) [Unconjugated] Summary

• Immunogen: Carcinogen-treated rat nodular hepatocytes
• Specificity: Detects human and rat OV-6 antigen.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OV-6 Antibody (OV-6) [Unconjugated]

• OV6
• OV-6

Background

OV-6 antigen has been used as a marker to identify oval cells, putative hepatic stem cells that can differentiate into multiple cell types.

1. Dunsford, H.A. and S. Sell (1989) Cancer Res. 49:4887.
2. Parent, R. et al. (2004) Gastroenterology 126:1147.
3. He, Z.P. et al. (2003) Cell Prolif. 37:177.
4. Lazaro, C.A. et al. (2003) Hepatology 38:1095.
5. He, Z.P. et al. (2003) Differentation 71:281.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OV-6 Antibody (MAB2020)(27)

Publications using MAB2020

• Abidov, M;Sokolova, K;Danilova, I;Baykenova, M;Gette, I;Mychlynina, E;Aydin Ozgur, B;Gurol, AO;Yilmaz, MT; Hepatic insulin synthesis increases in rat models of diabetes mellitus type 1 and 2 differently PloS one 2023-11-29 [PMID: 38019818] (IHC, Rat)
• MD Wang, H Xiang, L Zhang, C Wang Integration of OV6 expression and CD68+ tumor-associated macrophages with clinical features better predicts the prognosis of patients with hepatocellular carcinoma Translational Oncology, 2022-08-26;25(0):101509. 2022-08-26 [PMID: 36030750] (IHC, Human)
• ZQ Xie, HX Li, XJ Hou, MY Huang, ZM Zhu, LX Wei, CX Tang Capsaicin suppresses hepatocarcinogenesis by inhibiting the stemness of hepatic progenitor cells via SIRT1/SOX2 signaling pathway Cancer Medicine, 2022-06-08;0(0):. 2022-06-08 [PMID: 35674129] (IHC, Rat)
• Dan Liu, Hong Li, Hui Dong, Mincheng Qu, Liguang Yang, Lina Chen et al. Spatial Multiomics Analysis Reveals Only Minor Genetic and Epigenetic Changes in Human Liver Cancer Stem-Like Cells Compared With Other Tumor Parenchymal Cells Frontiers in Cell and Developmental Biology 2/9/2022 [PMID: 35223840]
• JY Ge, YW Zheng, T Tsuchida, K Furuya, H Isoda, H Taniguchi, N Ohkohchi, T Oda Hepatic stellate cells contribute to liver regeneration through galectins in hepatic stem cell niche Stem Cell Res Ther, 2020-09-29;11(1):425. 2020-09-29 [PMID: 32993816] (ICC, IHC, Rat)
• Y Zhao, J Zhu, B Shi, X Wang, Q Lu, C Li, H Chen The transcription factor LEF1 promotes tumorigenicity and activates the TGF-? signaling pathway in esophageal squamous cell carcinoma J. Exp. Clin. Cancer Res., 2019-07-11;38(1):304. 2019-07-11 [PMID: 31296250] (Flow Cytometry, IHC-P, Human, Xenograft)
• Y Zhao, Q Lu, C Li, X Wang, L Jiang, L Huang, C Wang, H Chen PRMT1 regulates the tumour-initiating properties of esophageal squamous cell carcinoma through histone H4 arginine methylation coupled with transcriptional activation Cell Death Dis, 2019-05-01;10(5):359. 2019-05-01 [PMID: 31043582] (Cell Sorting, IHC-P, Human, Xenograft)
• CS Chien, YH Chen, HL Chen, CP Wang, SH Wu, SL Ho, WC Huang, CH Yu, MH Chang Cells responsible for liver mass regeneration in rats with 2-acetylaminofluorene/partial hepatectomy injury J. Biomed. Sci., 2018-04-25;25(1):39. 2018-04-25 [PMID: 29695258] (IHC, Rat)
• S Damiati, M Peacock, S Leonhardt, L Damiati, MA Baghdadi, H Becker, R Kodzius, B Schuster Embedded Disposable Functionalized Electrochemical Biosensor with a 3D-Printed Flow Cell for Detection of Hepatic Oval Cells (HOCs) Genes (Basel), 2018-02-14;9(2):. 2018-02-14 [PMID: 29443890] (Cell Selection, Human)
• Hussein Abdellatif, Gamal Shiha, Dalia M. Saleh, Huda Eltahry, Kamal G. Botros Effect of human umbilical cord blood stem cell transplantation on oval cell response in 2-AAF/CCL4 liver injury model: experimental immunohistochemical study Inflammation and Regeneration 12/1/2017 [PMID: 29259704]
• JF Wu, HH Chang, MY Lu, ST Jou, KC Chang, YH Ni, MH Chang Prognostic roles of pathology markers immunoexpression and clinical parameters in Hepatoblastoma J. Biomed. Sci., 2017-08-29;24(1):62. 2017-08-29 [PMID: 28851352] (IHC-P, Human)
• C Wang, MD Wang, P Cheng, H Huang, W Dong, WW Zhang, PP Li, C Lin, ZY Pan, MC Wu, WP Zhou Hepatitis B virus X protein promotes the stem-like properties of OV6(+) cancer cells in hepatocellular carcinoma Cell Death Dis, 2017-01-19;8(1):e2560. 2017-01-19 [PMID: 28102846] (IHC, Human)
• DK DePeralta, L Wei, S Ghoshal, B Schmidt, GY Lauwers, M Lanuti, RT Chung, KK Tanabe, BC Fuchs Metformin prevents hepatocellular carcinoma development by suppressing hepatic progenitor cell activation in a rat model of cirrhosis Cancer, 2016-02-23;122(8):1216-27. 2016-02-23 [PMID: 26914713] (IHC, Rat)
• Olfat A. Hammam, Nagwa Elkhafif, Yasmeen M. Attia, Mohamed T. Mansour, Mohamed M. Elmazar, Rania M. Abdelsalam et al. Wharton’s jelly-derived mesenchymal stem cells combined with praziquantel as a potential therapy for Schistosoma mansoni-induced liver fibrosis Scientific Reports 2/15/2016 [PMID: 26876222]
• Olfat A. Hammam, Nagwa Elkhafif, Yasmeen M. Attia, Mohamed T. Mansour, Mohamed M. Elmazar, Rania M. Abdelsalam et al. Wharton’s jelly-derived mesenchymal stem cells combined with praziquantel as a potential therapy for Schistosoma mansoni-induced liver fibrosis Scientific Reports 2016-02-15 [PMID: 26876222] (Immunohistochemistry, Mouse)
• Xiao Zhang, Guangli Du, Ying Xu, Xuewei Li, Weiwei Fan, Jiamei Chen et al. Inhibition of notch signaling pathway prevents cholestatic liver fibrosis by decreasing the differentiation of hepatic progenitor cells into cholangiocytes Laboratory Investigation 3/1/2016 [PMID: 26692291]
• Chen Y, Chen H, Chien C, Wu S, Ho Y, Yu C, Chang M Contribution of Mature Hepatocytes to Biliary Regeneration in Rats with Acute and Chronic Biliary Injury. PLoS ONE, 2015-08-26;10(8):e0134327. 2015-08-26 [PMID: 26308208] (IHC, Rat)
• Chen J, Zhang X, Xu Y, Li X, Ren S, Zhou Y, Duan Y, Zern M, Zhang H, Chen G, Liu C, Mu Y, Liu P Hepatic Progenitor Cells Contribute to the Progression of 2-Acetylaminofluorene/Carbon Tetrachloride-Induced Cirrhosis via the Non-Canonical Wnt Pathway. PLoS ONE, 2015-06-18;10(6):e0130310. 2015-06-18 [PMID: 26087010] (IHC, Rat)
• Ji X, Xie Y, Zhong J, Xu Q, Zeng Q, Wang Y, Zhang Q, Shan Y GSK-3beta suppresses the proliferation of rat hepatic oval cells through modulating Wnt/beta-catenin signaling pathway. Acta Pharmacol Sin, 2015-02-09;36(3):334-42. 2015-02-09 [PMID: 25661318] (IHC-P, Rat)
• Zheng , Yun-Wen, Tsuchida , Tomonori, Shimao , Taiki, Li , Bin, Takebe , Takanori, Zhang , Ran-Ran, Sakurai , Yu, Ueno , Yasuharu, Sekine , Keisuke, Ishibashi , Naoto, Imajima , Makiko, Tanaka , Takuji, Taniguchi , Hideki The CD133+CD44+ precancerous subpopulation of oval cells is a therapeutic target for hepatocellular carcinoma. Stem Cells Dev, 2014-06-26;23(18):2237-49. 2014-06-26 [PMID: 24804872] (IHC-Fr, Rat)
• Banga , A, Greder , L V, Dutton , J R, Slack , J M W Stable insulin-secreting ducts formed by reprogramming of cells in the liver using a three-gene cocktail and a PPAR agonist. Gene Ther, 2013-10-03;21(1):19-27. 2013-10-03 [PMID: 24089243] (IHC, Mouse)
• Foteini Hassiotou, Anna R. Hepworth, Adriana S. Beltran, Michelle M. Mathews, Alison M. Stuebe, Peter E. Hartmann et al. Expression of the Pluripotency Transcription Factor OCT4 in the Normal and Aberrant Mammary Gland Frontiers in Oncology 3/23/2013 [PMID: 23596564]
• Yang Z, Wang L, Hou Y, Wang X Effects of matrine on oval cellmediated liver regeneration and expression of RBPJkappa and HES1. Mol Med Rep, 2013-03-27;7(5):1533-8. 2013-03-27 [PMID: 23545940] (IHC-P, Rat)
• Wang J, Koyota S, Zhou X, Ueno Y, Ma L, Kawagoe M, Koizumi Y, Okamoto H, Sugiyama T Expression and localization of regenerating gene I in a rat liver regeneration model. Biochem. Biophys. Res. Commun., 2009-01-27;380(3):472-7. 2009-01-27 [PMID: 19284990] (IHC-Fr, Rat)
• Yang W, Yan HX, Chen L, Liu Q, He YQ, Yu LX, Zhang SH, Huang DD, Tang L, Kong XN, Chen C, Liu SQ, Wu MC, Wang HY Wnt/beta-catenin signaling contributes to activation of normal and tumorigenic liver progenitor cells. Cancer Res., 2008-06-01;68(11):4287-95. 2008-06-01 [PMID: 18519688] (Flow Cytometry, Rat)
• Zhuang PY, Zhang JB, Zhu XD, Zhang W, Wu WZ, Tan YS, Hou J, Tang ZY, Qin LX, Sun HC Two pathologic types of hepatocellular carcinoma with lymph node metastasis with distinct prognosis on the basis of CK19 expression in tumor. Cancer, 2008-06-15;112(12):2740-8. 2008-06-15 [PMID: 18412155] (IHC-P, Human)
• Dezso K, Jelnes P, László V, Baghy K, Bödör C, Paku S, Tygstrup N, Bisgaard HC, Nagy P Thy-1 is expressed in hepatic myofibroblasts and not oval cells in stem cell-mediated liver regeneration. Am. J. Pathol., 2007-09-20;171(5):1529-37. 2007-09-20 [PMID: 17884967] (IHC-Fr, Rat)