OV-6 Antibody (OV-6) [Allophycocyanin]

Huh-7 human hepatoma cell line was stained with Mouse Anti-Human CD34 PE-conjugated Monoclonal Antibody (Catalog # FAB7227P) and either (A) Mouse Anti-Human/Rat OV-6 APC-conjugated Monoclonal Antibody (Catalog # FAB2020A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu, Rt
• Applications: Flow
• Clone: OV-6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

OV-6 Antibody (OV-6) [Allophycocyanin] Summary

• Immunogen: Carcinogen-treated rat nodular hepatocytes
• Specificity: Detects human and rat OV-6 antigen.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for OV-6 Antibody (OV-6) [Allophycocyanin]

• OV6
• OV-6

Background

OV-6 antigen has been used as a marker to identify oval cells, putative hepatic stem cells that can differentiate into multiple cell types.

1. Dunsford, H.A. and S. Sell (1989) Cancer Res. 49:4887.
2. Parent, R. et al. (2004) Gastroenterology 126:1147.
3. He, Z.P. et al. (2003) Cell Prolif. 37:177.
4. Lazaro, C.A. et al. (2003) Hepatology 38:1095.
5. He, Z.P. et al. (2003) Differentation 71:281.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OV-6 Antibody (FAB2020A)(3)

Publications using FAB2020A

• M Lee-Theile, JR Hadhoud, G Volante, DD Fadini, J Eichhorn, U Rolle, HC Fiegel Co-Expression of CD34, CD90, OV-6 and Cell-Surface Vimentin Defines Cancer Stem Cells of Hepatoblastoma, Which Are Affected by Hsp90 Inhibitor 17-AAG Cells, 2021-09-29;10(10):. 2021-09-29 [PMID: 34685577] (Flow Cytometry, Human)
• Y Zhao, J Zhu, B Shi, X Wang, Q Lu, C Li, H Chen The transcription factor LEF1 promotes tumorigenicity and activates the TGF-? signaling pathway in esophageal squamous cell carcinoma J. Exp. Clin. Cancer Res., 2019-07-11;38(1):304. 2019-07-11 [PMID: 31296250] (Flow Cytometry, Human)
• Y Zhao, Q Lu, C Li, X Wang, L Jiang, L Huang, C Wang, H Chen PRMT1 regulates the tumour-initiating properties of esophageal squamous cell carcinoma through histone H4 arginine methylation coupled with transcriptional activation Cell Death Dis, 2019-05-01;10(5):359. 2019-05-01 [PMID: 31043582] (Flow Cytometry, Human)