OspC Antibody

Images

 
Western Blot: OspC Antibody [NBP2-21683] - Detection of 0.1 ug of recombinant OspC protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspC fusion protein. Lane 3: MBP alone. Protein was run on a 4-20% gel, then ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

OspC Antibody Summary

Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi OspC protein. Protein accession number NP_047005.1.
Specificity
Cross-adsorbed against MBP from monospecific antiserum by chromatography. This antibody is specific for Borrelia burgdorferi OspC protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BB_B19
Purity
Ion exchange chromatography
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:2000
Application Notes
Tested for use in Western blotting and in ELISA. Expect a band approximately 20.7 kDa in size corresponding to Borrelia burgdorferi OspC protein by Western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Reactive in Borrelia burgdorferi. A BLAST analysis was used to suggest cross-reactivity with p39 from B. burgdorferi, afzelii, and valaisiana sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against B. japonica and americana sources based on 94% homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for OspC Antibody

  • ospC
  • BB_B19
  • Outer surface protein C
  • pc

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional OspC Products

OspC NBP2-21683

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Bioinformatics

Gene Symbol BB_B19