Nucleoli Marker Antibody (NM95) [Biotin]

Immunohistochemistry-Paraffin: Nucleoli Marker Antibody (NM95) [Biotin] [NBP2-34695B] - Human ovarian cancer cell line, staining of nucleoli. Antibody at 1:150. Image from verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal Nucleoli Marker Antibody (NM95) [Biotin] [NBP2-34695B] - Nucleoli Marker Antibody (NM95) immunoreactivity in adipose surrounding mouse kidney. The antibody was diluted 1 to 100 and left on tissue sections for 16h at 4 degrees Celsius. Image from a verified customer review.

• Reactivity: Hu, Bv(-), Mu(-), Rt(-)
• Applications: Flow, ICC/IF, IHC
• Clone: NM95
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

Nucleoli Marker Antibody (NM95) [Biotin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Nuclei of myeloid leukemia biopsy cells
• Localization: Nucleoli
• Marker: Marker For Human Cells
• Specificity: This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. monoclonal antibody NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Does not react with Mouse, Rat or Bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Nucleoli Marker Antibody (NBP2-34695B) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 03/18/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: PBMC
• Species: Human
• Lot: D158542

Comments

• Comments: Used Target Retrieval Solution preheated to 95 degrees Celsius. Total retrieval time was 20m in a vegetable steamer followed by a 15m cooldown period.

reviewed by: Faye Doherty IHC-P Human 07/01/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human ovarian cancer cell lines
• Species: Human
• Lot: MSM2-95PABX181203-060419-B

Comments

• Comments: Tested in IHC application, turned out excellent without any background noise using 1:150 dilution.