Western blot shows recombinant SARS-CoV-2 Nucleocapsid protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-SARS-CoV-2 Nucleocapsid Monoclonal Antibody (Catalog # MAB10474) followed by HRP-conjugated Anti-Mouse ...read more
SARS-CoV-2 Nucleocapsid was detected in immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung (left, positive staining) and normal human lung (right, negative control) using Mouse Anti-SARS-CoV-2 ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Nucleocapsid Antibody (1035111) [Unconjugated]
N Protein
Nucleocapsid protein
Nucleocapsid
ORF9a protein
Background
SARS-CoV-2,
which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs
to a family of viruses known as coronaviruses that are commonly comprised of
four structural proteins: Spike protein (S), Envelope protein (E), Membrane
protein (M), and Nucleocapsid protein (N) (1).
While the S, E and M proteins build up the viral envelop, the N protein is
involved transcription, replication and packaging of the viral RNA genome into
a helical ribonucleocapsid (RNP) (2, 3).
The SARS-CoV-2 N protein is a ~45 kDa protein composed of two
independent structural domains connected by a linker region. The N-terminal
region contains an RNA binding domain, the linker region interacts with the M
protein and the C-terminal region contains a self-association domain (2,3). The
SARS-CoV2 N protein shares 91% and 47% amino acid sequence identity with
SARS-CoV-1 and MERS N protein, respectively. The SARS-CoV-2 N protein displays VSR (viral
suppressor of RNA interference) activity in mammalian cells (4). In addition, the
N protein is an abundant protein during coronavirus infection and displays high
immunogenic activity (5, 6), so it has been used to develop serological diagnostic kit for
Covid-19 IgM and IgG antibody tests (7).
Wu, F. et al. (2020) Nature 579:265.
Chang, C. K. et al. (2006) J. Biomed. Sci. 13:59.
Hurst, K. R. et al. (2009) J. Virol. 83:7221.
Mu, J. et al. (2020) Sci. China Life Sci. doi: 10.1007/s11427-020-1692-1.
Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
Liu, W. et al. (2020) J. Clin. Microbiol. doi: 10.1128/JCM.00461-20.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Nucleocapsid Antibody (MAB10474)(9)
We have publications tested in 3 confirmed species: Human, Hamster, Hamster - Mesocricetus auratus (Golden Hamster).
We have publications tested in 4 applications: IHC, Immunohistochemistry, Neutralization, Western Blot.
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