![Western Blot: MuERVL-Gag Antibody [NBP2-66963] - Mouse embryonic stem cell (ESC) lysate. Antibody at 1:1000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MuERVL-Gag-Antibody-Western-Blot-NBP2-66963-img0002.jpg "Western Blot: MuERVL-Gag Antibody [NBP2-66963] - Mouse embryonic stem cell (ESC) lysate. Antibody at 1:1000. WB image submitted by a verified customer review.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide within human MuERVL-Gag aa 541-581/581. (SwissProt: V9H130 Mouse) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.2% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-66963 | Applications | Species |
|---|---|---|
| Detraux D, Caruso M, Feller L et Al. A critical role for heme synthesis and succinate in the regulation of pluripotent states transitions Elife 2023-07-10 [PMID: 37428012] |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Sergio de la Rosa |
Immunofluorescence and Western blot | Mouse | 01/07/2020 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Sergio de la Rosa 01/07/2020 |
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| Application: | Immunofluorescence and Western blot | |
| Species: | Mouse |
![Immunocytochemistry/Immunofluorescence: MuERVL-Gag Antibody [NBP2-66963] - Staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at 4C with MuERVL-Gag rabbit pAb at a 1:100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).](http://images.novusbio.com/fullsize/MuERVL-Gag-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-66963-img0001.jpg "Immunocytochemistry/Immunofluorescence: MuERVL-Gag Antibody [NBP2-66963] - Staining MuERVL-Gag in mouse 3-cell stage embryo (red). The cells were incubated overnight at 4C with MuERVL-Gag rabbit pAb at a 1:100 dilution (shown in red) and phalloidin (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).")
![Immunocytochemistry/Immunofluorescence: MuERVL-Gag Antibody [NBP2-66963] - Left panel: Mouse embryonic stem cells (mESCs) with antibody at 1:300 dilution. Right panel: Mouse 2-cell embryo with antibody at 1:100 dilution. ICC/IF images submitted by a verified customer review.](http://images.novusbio.com/fullsize/MuERVL-Gag-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-66963-img0003.jpg "Immunocytochemistry/Immunofluorescence: MuERVL-Gag Antibody [NBP2-66963] - Left panel: Mouse embryonic stem cells (mESCs) with antibody at 1:300 dilution. Right panel: Mouse 2-cell embryo with antibody at 1:100 dilution. ICC/IF images submitted by a verified customer review.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
