MRPS5 Antibody - BSA Free

Western Blot: MRPS5 Antibody [NBP2-98525] - Anti-MRPS5 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 45 kDa

Immunocytochemistry/Immunofluorescence: MRPS5 Antibody [NBP2-98525] - Immunofluorescence staining of MRPS5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MRPS5 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: MRPS5 Antibody [NBP2-98525] - Immunochemical staining of human MRPS5 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: MRPS5 Antibody [NBP2-98525] - Immunochemical staining of human MRPS5 in human gallbladder with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: MRPS5 Antibody [NBP2-98525] - MRPS5 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 ul anti-MRPS5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MRPS5 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 43 kDa

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPS5 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human MRPS5 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPS5
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MRPS5 Antibody - BSA Free

• mitochondrial 28S ribosomal protein S5
• mitochondrial ribosomal protein S5
• MRP-S5S5mt28S ribosomal protein S5, mitochondrial

Background

MRPS5, or Mitochondrial Ribosomal Protein S5, contains a long 48 kDa isoform and a short 28 kDa isoform, and is involved in protein synthesis as a component of the 28S small mitochondrial ribosome subunit. Current research is being conducted on MRPS5 and its relation to several diseases and disorders, including mycobacterium tuberculosis, pneumonia, and tuberculosis. The protein interacts with MRPS2, ICT1, ESR2, DDX56, and AGO1, and has been associated with the process of translation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRPS5 Antibody (NBP2-98525)(1)

Publication using NBP2-98525

• Zhu Z, Hou Q, Wang B et al. A novel mitochondria-related gene signature for controlling colon cancer cell mitochondrial respiration and proliferation Human cell [PMID: 35429326]

Bioinformatics

• Gene Symbol: MRPS5