MRPS10 Antibody

Images

 
Western Blot: MRPS10 Antibody [NBP2-19409] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MRPS10 Antibody [NBP2-19409] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MRPS10 Antibody [NBP2-19409] - Immunohistochemical analysis of paraffin-embedded U373 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1 mg/ml

Order Details

MRPS10 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MRPS10. The exact sequence is proprietary.
Localization
Mitochondrion
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MRPS10
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (88%).

Alternate Names for MRPS10 Antibody

  • 28S ribosomal protein S10, mitochondrial
  • FLJ10567
  • mitochondrial 28S ribosomal protein S10
  • mitochondrial ribosomal protein S10
  • MRP-S10
  • PNAS-122
  • S10mt

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MRPS10 Antibody (NBP2-19409) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MRPS10 Antibody Products

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Diseases for MRPS10 Antibody (NBP2-19409)

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Blogs on MRPS10

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MRPS10
Uniprot

Customer Resources

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