MRPS10 Antibody - BSA Free

Western Blot: MRPS10 Antibody [NBP2-19409] - Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: MRPS10 Antibody [NBP2-19409] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.

Immunohistochemistry: MRPS10 Antibody [NBP2-19409] - MRPS10 antibody [N1C3] detects MRPS10 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: MRPS10 antibody [N1C3] (NBP2-19409) dilution: 1:250.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPS10 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MRPS10. The exact sequence is proprietary.
• Localization: Mitochondrion
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPS10
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MRPS10 Antibody - BSA Free

• 28S ribosomal protein S10, mitochondrial
• FLJ10567
• mitochondrial 28S ribosomal protein S10
• mitochondrial ribosomal protein S10
• MRP-S10
• PNAS-122
• S10mt

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MRPS10
• Uniprot:
  • P82664(Human)