Mitochondria Antibody (113-1) - IHC-Prediluted

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - IHC-Prediluted [NBP2-45195] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with mitochondria monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - IHC-Prediluted [NBP2-45195] - Formalin-fixed paraffin-embedded human pancreas stained with mitochondria monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - IHC-Prediluted [NBP2-45195] - Analysis using the Biotin conjugate of this antibody. Staining of human prostate cancer tissue (left) and mouse stomach tissue (right). Image from verified customer review.

Immunohistochemistry-Paraffin: Mitochondria Antibody (113-1) - IHC-Prediluted [NBP2-45195] - Formalin-fixed paraffin-embedded human Renal cell carcinoma stained with Mitochondria Monoclonal antibody (113-1). Image using the Azide and BSA Free form of this antibody.

• Reactivity: Hu, Mu(-), Rt(-)
• Applications: IHC
• Clone: 113-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Mitochondria Antibody (113-1) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Semi-Purified mitochondrial preparation
• Localization: Mitochondria in cytoplasm
• Marker: Marker for Human Cells, Granular RCC s & Salivary Tumor
• Specificity: This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial monoclonal antibody has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this monoclonal antibody facilitates the classification of salivary tumors.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Mouse or Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Background

NB600-556 recognizes a 60kDa non-glycosylated protein component of mitochondria found in human cells. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker of the mitochondria in subcellular fractions. This monoclonal antibody is part of a new panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb-2 produces a spaghetti-like staining pattern in the cytoplasm of normal and malignant human cells and may be used to stain the mitochondria in paraformaldehyde fixed frozen and formalin-fixed, paraffin-embedded tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.