![Immunoprecipitation: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - C20orf72 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 µL NBP3-23534 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-23534,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :35 kDa](http://images.novusbio.com/fullsize/nbp3-23534_rabbit-mgme1-pab-azide-and-bsa-free-136202422345035.png "Immunoprecipitation: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - C20orf72 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 µL NBP3-23534 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-23534,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa Observed band size :35 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. |
| Immunogen | Produced in rabbits immunized with E. coli-derived Human MGME1 fragment |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MGME1 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MGME1 |
![Immunohistochemistry-Paraffin: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - Immunochemical staining of human MGME1 in human kidney with NBP3-23534 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23534_rabbit-mgme1-pab-azide-and-bsa-free-13620242234119.png "Immunohistochemistry-Paraffin: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - Immunochemical staining of human MGME1 in human kidney with NBP3-23534 at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Western Blot: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - NBP3-23534 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 35 kDa](http://images.novusbio.com/fullsize/nbp3-23534_rabbit-mgme1-pab-azide-and-bsa-free-136202422345030.png "Western Blot: MGME1 Antibody - Azide and BSA Free [NBP3-23534] - NBP3-23534 at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 39 kDa. Observed band size: 35 kDa")
![Immunohistochemistry-Paraffin: MGME1 Antibody [NBP3-23534] - Immunochemical staining of human MGME1 in human rectum with NBP3-23534 at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp3-23534_rabbit-mgme1-pab-azide-and-bsa-free-23520251664725.png "Immunohistochemistry-Paraffin: MGME1 Antibody [NBP3-23534] - Immunochemical staining of human MGME1 in human rectum with NBP3-23534 at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunocytochemistry/ Immunofluorescence: MGME1 Antibody [NBP3-23534] - Immunofluorescence staining of MGME1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-23534 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-23534_rabbit-mgme1-pab-azide-and-bsa-free-235202511545636.png "Immunocytochemistry/ Immunofluorescence: MGME1 Antibody [NBP3-23534] - Immunofluorescence staining of MGME1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-23534 (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")