![Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Western Blot analysis of Rat brain membrane lysate showing detection of mGluR5/1a Glutamate Receptor protein using Mouse Anti-mGluR5/1a Glutamate Receptor Monoclonal Antibody, Clone S75-33 (NBP2-59334). Primary Antibody: Mouse Anti-mGluR5/1a Glutamate Receptor Monoclonal Antibody (NBP2-59334) at 1:1000.](http://images.novusbio.com/fullsize/mGluR5-1a-Antibody-S75-33-Western-Blot-NBP2-59334-img0005.jpg "Western Blot: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Western Blot analysis of Rat brain membrane lysate showing detection of mGluR5/1a Glutamate Receptor protein using Mouse Anti-mGluR5/1a Glutamate Receptor Monoclonal Antibody, Clone S75-33 (NBP2-59334). Primary Antibody: Mouse Anti-mGluR5/1a Glutamate Receptor Monoclonal Antibody (NBP2-59334) at 1:1000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | S75-33 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Mouse mGluR5/1a Antibody (S75-33) - BSA Free (NBP2-59334) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b |
| Localization | Cell Membrane |
| Specificity | Detects 130kDa (both mGluR1 and mGluR5). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-33 (NBP2-59334). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (NBP2-59334) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) mGluR1/5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/mGluR5-1a-Antibody-S75-33-Immunocytochemistry-Immunofluorescence-NBP2-59334-img0006.jpg "Immunocytochemistry/Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-mGluR1/5 Monoclonal Antibody, Clone S75-33 (NBP2-59334). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody (NBP2-59334) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) mGluR1/5 Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/mGluR5-1a-Antibody-S75-33-Immunocytochemistry-Immunofluorescence-NBP2-59334-img0002.jpg "Immunocytochemistry/Immunofluorescence: mGluR5/1a Antibody (S75-33) [NBP2-59334] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-mGluR1/5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm, Nucleus. Magnification: 60X.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")