Methyl-Lysine Antibody - BSA Free

• Reactivity: All-NA
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Methyl-Lysine Antibody - BSA Free Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using methylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity-purified Methyl-Lysine Antibody was prepared from whole rabbit serum produced by repeated immunizations with Methyl-Lysine conjugated KLH.
• Specificity: Reactivity is specific for Methylated Lysine residues (mono, di).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:20000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
• Application Notes: This affinity-purified antibody is suitable for use in ELISA, immunohistochemistry, western blotting and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, and flow cytometry.

Reactivity Notes

The cross reactivity with tri-methyl lysine has not been tested. There are no cross reactions with acetylated proteins

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0, 50% (v/v) Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Methyl-Lysine Antibody - BSA Free

• Dimethylysine
• Methyl lysine
• methyllysine
• monomethyl lysine
• N epsilon dimethyl lysine
• N epsilon mono methyllysine
• N epsilon trimethyl lysine
• N(epsilon) trimethyl lysine
• Trimethyl lysine

Background

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.