Methyl-Lysine Antibody - Azide and BSA Free

Western Blot: Methyl-Lysine Antibody [NB600-824] - Western blot analysis of Bovine serum albumin showing detection of Methyl-Lysine protein using Rabbit Anti-Methyl-Lysine Polyclonal Antibody (NB600-824). Primary Antibody: Rabbit Anti-Methyl-Lysine Polyclonal Antibody (NB600-824) at 1:1000. Methyl-Lysine in BSA (Left) and Methylated BSA (Right).

Immunocytochemistry/Immunofluorescence: Methyl-Lysine Antibody [NB600-824] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody at 1:50 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Methylated Lysine Antibody. (C) Composite.

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Methyl-Lysine Antibody - Azide and BSA Free Summary

• Immunogen: Methylated KLH Conjugated
• Specificity: Detects proteins containing methylated lysine residues.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000-1:5000
• Application Notes: 0.2-0.5 ug/mL of Methyl-Lysine Antibody was sufficient for detection of the methylated histone by Western blot analysis using melanoma cells in TBSt.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: PBS, 50% Glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Methyl-Lysine Antibody - Azide and BSA Free

• Dimethylysine
• Methyl lysine
• methyllysine
• monomethyl lysine
• N epsilon dimethyl lysine
• N epsilon mono methyllysine
• N epsilon trimethyl lysine
• N(epsilon) trimethyl lysine
• Trimethyl lysine

Background

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Methyl-Lysine Antibody (NB600-824)(4)

Publications using NB600-824

• Dukic T, Smiljanic K, Mihailovic J et al. Proteomic Profiling of Major Peanut Allergens and Their Post-Translational Modifications Affected by Roasting Foods (Basel, Switzerland) 2022-12-09 [PMID: 36553735] (WB)
• Walker C, Batan D, Bishop C et al. Extracellular matrix stiffness controls cardiac valve myofibroblast activation through epigenetic remodeling Bioengineering & Translational Medicine 2022-08-22 [PMID: 36176599] (ICC/IF, Porcine)
  
  Details:
  Dilution used for ICC 1:500
• Roberts JL, Liu G, Paglia DN et al. Deletion of Wnt5a in osteoclasts results in bone loss through decreased bone formation Annals of the New York Academy of Sciences 2020-03-01 [PMID: 31919867] (Immunoprecipitation, Western Blot, Human)
• Bao L, Chen Y, et al. Methylation of hypoxia-inducible factor (HIF)-1 alpha by G9a/GLP inhibits HIF-1 transcriptional activity and cell migration. Nucleic Acids Res 2018-07-27 [PMID: 29860315] (WB, IP, Human)

FAQs for Methyl-Lysine Antibody (NB600-824). (Showing 1 - 1 of 1 FAQs).

1. I am interested in your anti-methylated lysine antibody (catalog # NB600-824). Could you kindly tell me which type of methylated lysine is recognized by the antibody, monomethyl, dimethyl or trimethyl, or all.
   • This product recognizes mono-methyl and di-methyl, but has not yet been tested for tri-methyl.