Metallothionein-1A Antibody (UC1MT)

Images

 
Western Blot: metallothionein Antibody (UC1MT) [NBP1-97493] - Lane 1: MW marker, Lane 2: MT I, Lane 3: MT II, Lane 4: Mummichog CdCl2.
Immunohistochemistry-Paraffin: metallothionein Antibody (UC1MT) [NBP1-97493] - Human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Eq, Fi, RbSpecies Glossary
Applications WB, EIA, IHC, IHC-P
Clone
UC1MT
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Metallothionein-1A Antibody (UC1MT) Summary

Immunogen
Cross-linked rabbit liver Metallothionein I and II.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MT1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Enzyme Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunohistochemistry-Paraffin and detects a band of ~15kDa by Western blot.
Publications
Read Publications using
NBP1-97493 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for Metallothionein-1A Antibody (UC1MT)

  • metallothionein 1A (functional)
  • metallothionein 1A
  • metallothionein 1S
  • metallothionein-1A
  • metallothionein-IA
  • MGC32848
  • MT1
  • MT-1A
  • MT1S
  • MTC
  • MT-IA

Background

Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for metallothionein Antibody (NBP1-97493)(4)

Reviews for metallothionein Antibody (NBP1-97493) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for metallothionein Antibody (NBP1-97493). (Showing 1 - 3 of 3 FAQs).

  1. Can you please confirm whether the following item can be recommended for IF?
    • I can confirm that this antibody has not been tested for use in IF. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.
  2. Our customer would like to perform ELISA with NBP1-97493. Would you please help suggest the suitable protein product (Human preferred) to work with NBP1-97493 as the positive control or standard?
    • This antibody reacts to Metallothionein 1 and 2. Any of these Metallothionein 1 or 2 recombinant proteins should be suitable as a positive control.
  3. Would you be able to give me details about how the example blot on the datasheet was run? (what percentage gel? what type of gel? what type of block?)
    • Indicated amounts of protein were denatured by boiling in 10% SDS buffer (10% w/v SDS; 0.5 M Tris-HCL, pH 6.8, 5% [v/v] 2-mercaptoethanol; 5% [v/v] glycerol), resolved by electrophoresis on 12% SDS-polyacrylamide gels and transferred (60 V for a half hour in 12 mM Tris-HCL, 96 mM glycine, 15% methanol) to nitrocellulose filters. The resulting blots were blocked overnight in Superblock (Pierce, Rock-ford, IL). The blot was washed three times with PBS over 15 minutes, incubated with a 1:200 dilution of mouse anti-MT antibody, and washed three times in PBS containing 0.1% Tween 20. Immunoreactive MT was visualized using ECL Western blotting reagents (Amersham-Pharmacia, Piscataway, NJ).

Secondary Antibodies

 

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Bioinformatics

Gene Symbol MT1A