Metallothionein-1A Antibody (UC1MT)

Images

 
Western Blot: metallothionein Antibody (UC1MT) [NBP1-97493] - Lane 1: MW marker, Lane 2: MT I, Lane 3: MT II, Lane 4: Mummichog CdCl2.
Immunohistochemistry-Paraffin: metallothionein Antibody (UC1MT) [NBP1-97493] - Human uterus tissue stained with Metallothionein, mAb (UC1MT) at 10ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Eq, Fi, RbSpecies Glossary
Applications WB, EIA, IHC, IHC-P
Clone
UC1MT
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Metallothionein-1A Antibody (UC1MT) Summary

Immunogen
Cross-linked rabbit liver Metallothionein I and II.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MT1A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Enzyme Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
Application Notes
Immunohistochemistry-Paraffin and detects a band of ~15kDa by Western blot.
Publications
Read Publications using
NBP1-97493 in the following applications:

  • 1 publication
  • WB
    4 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for Metallothionein-1A Antibody (UC1MT)

  • metallothionein 1A (functional)
  • metallothionein 1A
  • metallothionein 1S
  • metallothionein-1A
  • metallothionein-IA
  • MGC32848
  • MT1
  • MT-1A
  • MT1S
  • MTC
  • MT-IA

Background

Metallothioneins (MTs) are a class of ubiquitous, low molecular weight, cysteine-rich, metal binding proteins that function in the metabolism of zinc and copper. MT protects against exposure to toxic metals such as mercury and cadmium as well as a variety of other stress conditions. There are four mammalian MT isoforms: MT-I, -II, -III, -IV, each of which is encoded by individual genes. MT expression is induced by heavy metal cations, starvation, elevated temperature, lipopolysaccharide, inflammatory cytokines, free radicals, and ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF482
Species: Mu
Applications: IHC, WB
H00004502-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, WB
NLS932
Species: Ch, Hu, Mu, Rt
Applications: IHC, IHC-Fr, IHC-P, KD, WB
NB120-14817
Species: Hu, Rt
Applications: ICC/IF, IHC, S-ELISA, WB
H00027349-M01
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
NBP2-67415
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-82991
Species: Hu
Applications: IHC, IHC-P, WB
MMP200
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
NBP1-89772
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
7268-CT
Species: Hu
Applications: BA
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-34594
Species: Hu, Pm
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P
AF4478
Species: Hu
Applications: IHC, WB
H00005670-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
DTM100
Species: Hu
Applications: ELISA
NBP2-37447
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IF, IHC, IHC-P, WB
NBP1-97493
Species: Hu, Mu, Rt, Eq, Fi, Rb
Applications: WB, EIA, IHC, IHC-P

Publications for metallothionein Antibody (NBP1-97493)(7)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(2)
Rabbit
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-97493 Applications Species
Han H, Nakaoka HJ, Hofmann L et al. The Hippo pathway kinases LATS1 and LATS2 attenuate cellular responses to heavy metals through phosphorylating MTF1 Nature cell biology Jan 1 2022 [PMID: 35027733]
Kim JH, Jeon J, Shin M et al. Regulation of the Catabolic Cascade in Osteoarthritis by the Zinc-ZIP8-MTF1 Axis. Cell 2014 Feb 17 [PMID: 24529376] (IHC-P, Mouse) IHC-P Mouse
SG Kim et al. Potentiation of cadmium-induced cytotoxicity by sulfur amino acid deprivation through activation of extracellular signal-regulated kinase1/2 (ERK1/2) in conjunction with p38 kinase or c-jun N-terminal kinase (JNK). Complete inhibition of the potentiated t. Biochem. Pharmacol. 62, 1379. 2001 [PMID: 11709198] (WB, Rat) WB Rat
M Kantorow et al. Identification and spatial analysis of metallothioneins expressed by the adult human lens. Invest. Ophthalmol. Vis. Sci. 42, 188. 2001 [PMID: 11133866] (WB, Human) WB Human
EA Olson et al. Immunomodulatory activities of extracellular metallothionein. I. Metallothionein effects on antibody production. Toxicology 85, 161 . 1993 [PMID: 8303711] (WB, Rabbit) WB Rabbit
Gumasta P Immunohistochemical Expression of Oxidative Stress Markers in Bovine Tissues Correlated to Cadmium Concentration JAR Oct 15 2019
Chun K, Lee C, Kim K et al. Effects of Androgen Receptor Inhibition on Kanamycin-Induced Hearing Loss in Rats International Journal of Molecular Sciences May 18 2021 [PMID: 34070066] (WB, Rat) WB Rat

Reviews for metallothionein Antibody (NBP1-97493) (0)

There are no reviews for metallothionein Antibody (NBP1-97493). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for metallothionein Antibody (NBP1-97493). (Showing 1 - 3 of 3 FAQs).

  1. Can you please confirm whether the following item can be recommended for IF?
    • I can confirm that this antibody has not been tested for use in IF. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.
  2. Our customer would like to perform ELISA with NBP1-97493. Would you please help suggest the suitable protein product (Human preferred) to work with NBP1-97493 as the positive control or standard?
    • This antibody reacts to Metallothionein 1 and 2. Any of these Metallothionein 1 or 2 recombinant proteins should be suitable as a positive control.
  3. Would you be able to give me details about how the example blot on the datasheet was run? (what percentage gel? what type of gel? what type of block?)
    • Indicated amounts of protein were denatured by boiling in 10% SDS buffer (10% w/v SDS; 0.5 M Tris-HCL, pH 6.8, 5% [v/v] 2-mercaptoethanol; 5% [v/v] glycerol), resolved by electrophoresis on 12% SDS-polyacrylamide gels and transferred (60 V for a half hour in 12 mM Tris-HCL, 96 mM glycine, 15% methanol) to nitrocellulose filters. The resulting blots were blocked overnight in Superblock (Pierce, Rock-ford, IL). The blot was washed three times with PBS over 15 minutes, incubated with a 1:200 dilution of mouse anti-MT antibody, and washed three times in PBS containing 0.1% Tween 20. Immunoreactive MT was visualized using ECL Western blotting reagents (Amersham-Pharmacia, Piscataway, NJ).

Secondary Antibodies

 

Isotype Controls

Additional Metallothionein-1A Products

Bioinformatics Tool for metallothionein Antibody (NBP1-97493)

Discover related pathways, diseases and genes to metallothionein Antibody (NBP1-97493). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for metallothionein Antibody (NBP1-97493)

Discover more about diseases related to metallothionein Antibody (NBP1-97493).
 

Pathways for metallothionein Antibody (NBP1-97493)

View related products by pathway.

PTMs for metallothionein Antibody (NBP1-97493)

Learn more about PTMs related to metallothionein Antibody (NBP1-97493).
 

Research Areas for metallothionein Antibody (NBP1-97493)

Find related products by research area.

Blogs on Metallothionein-1A

There are no specific blogs for Metallothionein-1A, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Metallothionein-1A Antibody (UC1MT) and receive a gift card or discount.

Bioinformatics

Gene Symbol MT1A