MARCH10 Antibody

Western Blot: MARCH10 Antibody [NBP1-28483] - (0.3ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: MARCH10 Antibody [NBP1-28483] - (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Rt, Hu, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MARCH10 Antibody Summary

• Immunogen: Peptide with sequence C-SRNYPQPRTEE corresponding to internal region according to NP_001094345.1, NP_689811.2.
• Epitope: C terminus
• Specificity: This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.
• Predicted Species: Human (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MARCH10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:100-1:2000
• Application Notes: WB: Approx. 90 kDa band observed in rat testis lysates (calculated MW of 90.5 kDa band according to human NP_689811.2 and of 87.9 kDa band according to mouse NP_766156.2). An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MARCH10 Antibody

• EC 6.3.2.-
• FLJ35757
• MARCH-Xprobable E3 ubiquitin-protein ligase MARCH10
• membrane-associated ring finger (C3HC4) 10
• Membrane-associated RING finger protein 10
• Membrane-associated RING-CH protein X
• RING finger protein 190RNF190

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MARCH10 Antibody (NBP1-28483)(1)

Publication using NBP1-28483

• Morokuma Y, Nakamura N, Kato A et al. MARCH-XI, a novel transmembrane ubiquitin ligase implicated inubiquitin-dependent protein sorting in developing spermatids. J Biol Chem 2007-08-24 [PMID: 17604280]

Bioinformatics

• Gene Symbol: MARCH10
• Entrez:
  • 162333(Human)
  • 303596(Rat)
• Uniprot:
  • NP_001094345.1(Human)