Malondialdehyde Antibody (11E3)

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde -BSA using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3.Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 ug). Lane 3: Malondialdehyde-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Immunocytochemistry/Immunofluorescence: Malondialdehyde Antibody (11E3) [NBP2-59367] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). Courtesy of: Dr. Robert Burke, University of Victoria.

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Analysis of Human Cervical Cancer cell line (HeLa) showing detection of Malondialdehyde -BSA using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.

• Reactivity: NA
• Applications: WB, ELISA, ICC/IF
• Clone: 11E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Malondialdehyde Antibody (11E3) Summary

• Immunogen: Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS pH 7.4, 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.