mAID Tag Antibody

Western Blot: mAID Tag Antibody [NBP2-89163] - Analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.

• Reactivity: All-NA
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

mAID Tag Antibody Summary

• Immunogen: Synthetic peptide immune sequence is PKDPAKPPAKAQVVGWPC.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for mAID Tag Antibody

• Auxin-inducible degron
• mini-AID-tag

Background

Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mAID Tag Antibody (NBP2-89163)(2)

Publications using NBP2-89163

• Oberlitner J, Tinman M, Das A et al. Analysis of rad-51 separation of function allele suggests divergence of the synthesis-dependent strand annealing and double Holliday junction pathways prior to RAD-51 filament disassembly Genetics 2025-06-01 [PMID: 40192500]
• Xu X, Huang Y, Yang F et al. NudCL2 is required for cytokinesis by stabilizing RCC2 with Hsp90 at the midbody Protein & Cell 2024-10-01 [PMID: 38801297]