MAGED4B Overexpression Lysate (Native)

Images

 
Western Blot: MAGED4B Overexpression Lysate (Adult Normal) [NBL1-12817] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAGED4B.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MAGED4B Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC212260 and based on accession number NM_177535. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens melanoma antigen family D, 4B (MAGED4B), transcript variant 2, mRNA.
Gene
MAGED4B

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
81.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MAGED4B

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MAGED4B Overexpression Lysate (Native)

  • KIAA1859FLJ39060
  • MAGE1
  • MAGE-D4 antigen
  • MAGE-E1 antigen
  • MAGE-E1
  • MAGEE1MAGED4A
  • melanoma antigen family D, 4
  • MGC3210

Background

This gene is a member of the MAGED gene family. It is expressed only in brain and ovary among normal tissues, and two transcript variants of this gene are specifically expressed in glioma cells among cancer cells. This gene and the other MAGED genes are clustered on chromosome Xp11. Multiple alternatively spliced transcript variants have been found for this gene, however, the full-length nature of some variants has not been defined. [provided by RefSeq]. Transcript Variant: This variant (2), also known as MAGE-E1a, lacks an internal segment in the 5' UTR, as compared to variant 1. Both variants 1 and 2 encode the same isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol MAGED4B