LMW E.Coli Host Cell Protein Antibody - BSA Free

Western Blot: LMW E.Coli Host Cell Protein Antibody [NBP2-44207] - 2D Western Blot of anti-E.coli Low Molecular Weight Host Cell Protein antibody. Load: 35 Ug LMW HCP.

ELISA: LMW E.Coli Host Cell Protein Antibody [NBP2-44207] - ELISA of Rabbit anti-Low Molecular Weight Host Cell Protein Antigen: BSA conjugates of LMW HCP. Coating amount: 1 Ug per well titrated 1:3 in 1XPBS.

2D Western Blot of LMW E.Coli Host Cell Protein Antibody. Load: 35 ug LMW HCP. Primary antibody: Rabbit a antibody at 1:200 for overnight at 4C. Secondary antibody: Goat anti-rabbit secondary antibody at 1:10,000 for 30 min at RT. Block for 1 hour at RT.

• Reactivity: Ec
• Applications: WB, DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LMW E.Coli Host Cell Protein Antibody - BSA Free Summary

• Description: LMW Host Cell Protein A purified antibody is directed against low molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography.
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: LMW E.Coli Host Cell Protein Antibody was prepared by repeated immunizations with a mixture low molecular weight (LMW) proteins from E.Coli (BL21).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:1000-1:2000
  • Western Blot 1:500-1:1000
• Application Notes: This product detects over 270 E.coli LMW proteins and has been tested by 2D-Page, western blotting, and ELISA.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.