Lambda Light Chain Antibody (RM127)

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody (RM127) [Unconjugated] [NBP2-62021] - Immunohistochemistry of Human Tonsil using NBP2-62021.

ELISA: Lambda Light Chain Antibody (RM127) - Azide and BSA Free [NBP2-62021] - ELISA of human immunoglobulins shows RM127 reacts to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA: Lambda Light Chain Antibody (RM127) - Azide and BSA Free [NBP2-62021] - A titer ELISA using RM127. The plate was coated with different amounts of human IgG3l. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC
• Clone: RM127
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Lambda Light Chain Antibody (RM127) Summary

• Description: Novus Biologicals Rabbit Lambda Light Chain Antibody (RM127) (NBP2-62021) is a recombinant monoclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human IgG
• Specificity: This antibody reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. This antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGLC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.05ug/ml - 0.2ug/ml
  • Flow Cytometry 0.2ug/ml-2ug/ml
  • Immunocytochemistry/ Immunofluorescence 0.5ug/ml - 2ug/ml
  • Immunohistochemistry 0.5ug/ml - 2ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Lambda Light Chain Antibody (RM127)

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGLC1