L-DOPA Antibody

Dot blot analysis of 100 ng BSA-conjugated levodopa, using levodopa antibody (NBP3-48640) at 1:4000, 1:8000, and 1:16000.

ELISA plate is coated with 100 ul of levodopa-carrier protein conjugate at 10 ug/mL. The coated ligand is detected with levodopa antibody (NBP3-48640) at concentrations ranging from 360 ng/mL down to 1.3 ng/mL. Rabbit IgG antibody (HRP) is diluted at 1:2,000 and used to detect the primary antibody.

• Reactivity: All-NA
• Applications: DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

L-DOPA Antibody Summary

• Description: Novus Biologicals Rabbit L-DOPA Antibody (NBP3-48640) is a polyclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen used to generate this antibody corresponds to Levodopa (L-DOPA)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for L-DOPA Antibody

• 3 4 dihydroxy L phenylalanine
• L dihydroxyphenylalanine
• Levodopa

Background

L Dopa is an intermediate in dopamine biosynthesis. Clinically, L Dopa is used in the management of Parkinson's disease. It is used as a prodrug to increase dopamine levels since it is able to cross the blood-brain barrier whereas dopamine itself cannot. Once L Dopa has entered the central nervous system (CNS), it is metabolised to dopamine by aromatic L amino acid decarboxylase. This also occurs in the peripheral tissues, causing adverse effects and decreasing the available dopamine to the CNS, so it is standard practice to co administer a peripheral DOPA decarboxylase inhibitor and often a catechol-O-methyl transferase (COMT) inhibitor. The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated L-DOPA antibodies gave a good staining between 1/2000-1/5000 dilution in these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.