L-Asparaginase Antibody

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Western Blot: L-Asparaginase Antibody [NBP1-78086] - Lane 1: L-Asparaginase. Lane 2: none. Load: 100 ug per lane. Primary antibody: L-Asparaginase antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) ...read more

Product Details

Summary
Reactivity EcSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

L-Asparaginase Antibody Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
L-L-Asparaginase [E. coli] (Uniprot: P00805)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Multi-step
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:25000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:3000
Application Notes
This antibody has been assayed in western blot and against 1.0 ug of Asparaginase [E. coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H+L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:400,000 is suggested for this product.
Theoretical MW
36.85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reactivity against L-Asparaginase from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 100 ul deionized water (or equivalent)

Notes

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Asparaginase [E.coli]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for L-Asparaginase Antibody

  • AnsA
  • AnsB
  • Asparaginase
  • Colaspase
  • Cytoplasmic asparaginase I
  • L ASNase I
  • L ASNase II
  • L asparaginase 1
  • L asparaginase 2
  • L asparaginase I
  • L asparaginase II precursor
  • L asparaginase II
  • L asparagine amidohydrolase I
  • L asparagine amidohydrolase II

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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