KDELR3 Antibody

Product Discontinued

KDELR3 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 71-120 of Human KDEL Receptor 3.
• Specificity: KDEL Receptor 3 (L95) detects endogenous levels of KDEL Receptor 3 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDELR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of KDEL Receptor 3 (L95) pAb in extracts from 3T3 cells.
  Immunohistochemistry (IHC) analyzes of KDEL Receptor 3 (L95) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KDELR3 Antibody

• ER lumen protein retaining receptor 3
• ERD2L3
• KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3
• KDEL endoplasmic reticulum protein retention receptor 3
• KDEL receptor 3

Background

Soluble proteins in the endoplasmic reticulum (ER) contain a specific carboxy terminal sequence KDEL (Lys-Asp-Glu-Leu), and include the coat proteins required for vesicle budding from the ER, proteins that form retrogradevesicles on post-ER compartments, and integral membrane proteins that target vesicles to their correct destination. The retention of these soluble proteins in the ER depends on the interaction of the KDEL sequence with the corresponding KDEL receptor, also designated ERD2, in the Golgi apparatus.When KDEL proteins reach the Golgi complex, they are recognized by the KDEL receptor and transported retrograde in COPI-coated vesicles back to the ER. The small GTPase ADP-ribosylation factor 1 (ARF1), a regulator of vesicle transport, interacts with the KDEL receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDELR3
• Entrez:
  • 11015(Human)