Katanin p60 Antibody

Western Blot: Katanin p60 Antibody [H00011104-B01P] - Analysis of KATNA1 expression in NIH/3T3.

Western Blot: Katanin p60 Antibody [H00011104-B01P] - Analysis of KATNA1 expression in PC-12.

Western Blot: Katanin p60 Antibody [H00011104-B01P] - Analysis of KATNA1 expression in Raw 264.7.

Western Blot: Katanin p60 Antibody [H00011104-B01P] - Analysis of KATNA1 expression in transfected 293T cell line by KATNA1 polyclonal antibody. Lane 1: KATNA1 transfected lysate(34.32 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Katanin p60 Antibody Summary

• Immunogen: KATNA1 (AAH50428.1, 1 a.a. - 311 a.a.) full-length human protein. MSLLMISENVKLAREYALLGNYDSAMVYYQGVLDQMNKYLYSVKDTYLQQKWQQVWQEINVEAKHVKDIMKTLESFKLDSTPLKAAQHDLPASEGEVWSMPVPVERRPSPGPRKRQSSQYSDPKSHGNRPSTTVRVHRSSAQNVHNDRGKAVRCREKKEQNKGREEKGVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYSPATIFIDEIDSICSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSGMRP
• Specificity: KATNA1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: KATNA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Katanin p60 Antibody

• EC 3.6.4.3
• katanin p60 (ATPase containing) subunit A 1
• katanin p60 (ATPase-containing) subunit A 1
• katanin p60 ATPase-containing subunit A1
• Katanin p60 subunit A1
• Katanin p60
• KATNA1
• p60 katanin

Background

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. This gene encodes the p80 subunit. This protein is a member of the AAA family of ATPases. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Katanin p60 Antibody (H00011104-B01P). (Showing 1 - 1 of 1 FAQ).

1. I have a question about your Katanin p60 antibody, cat# H00011104-B01P. Your data sheet indicates that it detects an about 36 kDa (transfected 293T) and a 50 kDa (NIH/3T3) protein species. Are these the 60 and 37 kDa isoforms of Katanin p60?
   • This antibody is raised against the shorter isoform 2 of this protein, O75449 (https://www.uniprot.org/uniprotkb/O75449/entry) It looks like isoform 2 is similar to isoform 1, but missing parts of the sequence. I believe from the tests on endogenous lysates this antibody can detect the larger isoform. In the transfected lysate the lysate was transfected with the smaller isoform 2 sequence, which is why it picks up the lower molecular weight band. This antibody should pick up both isoforms.

Bioinformatics

• Gene Symbol: KATNA1
• Entrez:
  • 11104(Human)
• Uniprot:
  • AAH50428.1(Human)