Katanin p60 Antibody (717705) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: IHC
• Clone: 717705
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

Katanin p60 Antibody (717705) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human Katanin p60
  Asp308-Tyr378
  Accession # O75449
• Specificity: Detects human Katanin p60 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Katanin p60 subunit A-like 2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Katanin p60 Antibody (717705) [Alexa Fluor® 750]

• EC 3.6.4.3
• katanin p60 (ATPase containing) subunit A 1
• katanin p60 (ATPase-containing) subunit A 1
• katanin p60 ATPase-containing subunit A1
• Katanin p60 subunit A1
• Katanin p60
• KATNA1
• p60 katanin

Background

KATNA1 (Katanin [Japanese word for sword] p60 ATPase-containing subunit A1; also Katanin p60) is a 55-60 kDa member of the VPS4 subfamily, AAA ATPase family of molecules. It is ubiquitously expressed and is recognized to sever microtubules, allowing for their reorganization during cell division and migration. KATNA1 is most effective on nonacetylated, non-tau binding microtubules. In general, KATNA1 activity is regulated by binding to KATNB1/katanin p80, which potentiates KATNA1 action. However, the relationship is complex, and governed by the local p60:p80 ratio. Human KATNA1 is 491 amino acids (aa) in length. It contains a p80 interaction segment (aa 1-29), followed by a microtubule interaction region (aa 30-185), an ATPase domain (aa 239-381), and an oligomerization region (aa 455-489). KATNA1 is phosphorylated on Ser170. There is one isoform variant that shows a deletion of aa 168-243 coupled to a four aa substitution for aa 384-491. Over aa 308-378, human KATNA1 shares 97% aa identity with mouse KATNA1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.