Influenza B nucleoprotein Antibody (016) - Azide and BSA Free

Western Blot: Influenza B nucleoprotein Antibody (016) [NBP3-12755] - Reocombinant Protein 10 ng

NBP3-12755 used at 1:2000 dilution. Lane 1: H1N1 (A/Brevig Mission/1/1918) NP. Lane 2: H1N1 (A/Brisbane/02/2018 NP. Lane 3: H1N1 (A/California/07/2009) NP. Lane 4: H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP. Lane 5: H1N1 (A/Hawaii/70/2019) NP. Lane 6: H1N1 (A/Michigan/45/2015 NP. Lane 7: H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M). Lane 8:H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019 NP. Lane 9: H2N2 (A/Ann Arbor/6/1960) NP. Lane 10: H3N2 (A/Aichi/2/1968) NP. Lane 11: H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP. Lane 12: H3N2 (A/Hong Kong/1/1968) NP. Lane 13: H3N2 (A/Hong Kong/2671/2019) NP. Lane 14: H3N2 (A/Hong Kong/45/2019) NP. Lane 15: H3N2 (A/Hong Kong/4801/2014 NP. Lane 16: H3N2 (A/Kansas/14/2017 NP. Lane 17: H3N2 (A/Switzerland/9715293/2013) NP. Lane 18: H7N9 (A/Anhui/1-BALF_RG6/2013) NP. Lane 19: H7N9 (A/Shanghai/2/2013) NP. Lane 20: Influenza B (B/Brisbane/60/2008 NP. Lane 21: Influenza B (B/Colorado/06/2017 NP. Lane 22: Influenza B (B/Florida/4/2006) NP. Lane 23: Influenza B (B/Phuket/3073/2013) NP. Lane 24: Influenza B (B/Washington/02/2019) NP. Secondary Goat Anti-Rabbit IgG (H+L)[HRP] used at at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.

Western Blot: Influenza B nucleoprotein Antibody (016) [NBP3-12755] - NBP3-12755 in 1ug/mL. Sample: Recombinant Protein 10 ng Lane 1: Influenza A H5N1 Nucleoprotein (A/Texas/37/2024) Lane 2: Influenza A H1N1 Nucleoprotein (A/Brevig Mission/1/1918) Lane 3: Influenza B Nucleoprotein (B/Phuket/3073/2013) Secondary Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions.

• Reactivity: V-Vi
• Applications: WB, ELISA
• Clone: 016
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Influenza B nucleoprotein Antibody (016) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Preservative-Free. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Influenza B (Accession#: ACF54251.1; Met1-Tyr560).
• Specificity: Widely recognize Influenza B Nucleoprotein
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.1-0.2 ug/mL
  • Western Blot 0.1-1 ug/mL

Reactivity Notes

Pan Influenza B

Has cross-reactivity in ELISA and WB with:
Influenza B (B/Brisbane/60/2008) NP
Influenza B (B/Colorado/06/2017) NP
Influenza B (B/Florida/4/2006) NP
Influenza B (B/Phuket/3073/2013) NP
Influenza B (B/Washington/02/2019) NP

No cross-reactivity in ELISA and WB with:
H1N1 (A/Brevig Mission/1/1918) NP
H1N1 (A/Brisbane/02/2018 NP
H1N1 (A/California/07/2009) NP
H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP
H1N1 (A/Hawaii/70/2019) NP
H1N1 (A/Michigan/45/2015 NP
H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M)
H1N1 (A/Victoria/2570/2019)/(A/Wisconsin/588/2019) NP
H2N2 (A/Ann Arbor/6/1960) NP
H3N2 (A/Aichi/2/1968) NP
H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP
H3N2 (A/Hong Kong/1/1968) NP
H3N2 (A/Hong Kong/2671/2019) NP
H3N2 (A/Hong Kong/45/2019) NP
H3N2 (A/Hong Kong/4801/2014) NP
H3N2 (A/Kansas/14/2017) NP
H3N2 (A/Switzerland/9715293/2013) NP
H7N9 (A/Anhui/1-BALF_RG6/2013) NP
H7N9 (A/Shanghai/2/2013) NP

No cross-reactivity in ELISA with:
SARS-CoV-2 Nucleocapsid Protein
HCoV-229E Nucleocapsid Protein
HCoV-NL63 Nucleocapsid Protein
HCoV-HKU1 Nucleocapsid Protein
HCoV-OC43 Nucleocapsid Protein

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Influenza B nucleoprotein Antibody (016) - Azide and BSA Free

• Influenza B Virus NP
• NP
• Nucleoprotein

Background

Influenza virus B is a genus in the virus family Orthomyxoviridae. A limited host range means that Influenza virus B pandemics are rare. The nucleoprotein protects the negative strand viral RNA from nucleases by encapsidating it. encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Influenza B nucleoprotein Antibody (NBP3-12755). (Showing 1 - 1 of 1 FAQ).

1. We ordered your Influenza A nucleoprotein. We tested it. And it worked well for us. I am wondering if you also have Influenza B nucleoprotein, Recombinant. I don’t see it in your catalog.
   • At this time, it appears we only carry antibodies direct towards Influenza B nucleoprotein. We do not currently have a recombinant protein to this target. I apologize for the inconvenience.