![Western Blot: Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569] - Anti-HA mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane A: H3N2 (A/Darwin/9/2021) HA Protein Lane B: H3N2 (A/Darwin/9/2021) HA ProteinLane C: H3N2 (A/Darwin/6/2021) HA ProteinLane D: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein Lane E: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA ProteinSecondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.](http://images.novusbio.com/fullsize/Influenza-B-Hemagglutinin-Antibody-10H6C8G4-Western-Blot-NBP3-06569-img0001.jpg "Western Blot: Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569] - Anti-HA mouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane A: H3N2 (A/Darwin/9/2021) HA Protein Lane B: H3N2 (A/Darwin/9/2021) HA ProteinLane C: H3N2 (A/Darwin/6/2021) HA ProteinLane D: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein Lane E: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA ProteinSecondary: Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 10H6C8G4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza B Hemagglutinin (Accession#: ACA33493.1; Met 1-Ala 555). |
| Specificity | Has cross-reactivity in ELISA with: Influenza B (B/Brisbane/60/2008) HA Influenza B (B/Colorado/06/2017) HA Influenza B (B/PHUKET/3073/2013) HA Influenza B (B/Washington/02/2019) HA No cross-reactivity in ELISA with: H1N1 (A/Brisbane/02/2018) HA H1N1 (A/Brisbane/59/2007) HA H1N1 (A/California/04/2009) HA H1N1 (A/California/07/2009) HA H1N1 (A/Guangdong-Maonan/SWL1536/2019) / (A/Hawaii/70/2019) HA H1N1 (A/Michigan/45/2015) HA H1N1 (A/Puerto Rico/8/1934) HA H1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HA H2N2 (A/Canada/720/2005) HA H3N2 (A/Brisbane/10/2007) HA H3N2 (A/Cambodia/e0826360/2020) HA H3N2 (A/Hong Kong/2671/2019) HA H3N2 (A/Hong Kong/45/2019) HA H3N2 (A/Hong Kong/4801/2014) HA H3N2 (A/Kansas/14/2017) HA H3N2 (A/Singapore/INFIMH-16-0019/2016) HA H3N2 (A/Switzerland/9715293/2013) HA H4N4 (A/mallard/Ohio/657/2002) HA H5N1 (A/Anhui/1/2005) HA H5N8 (A/northern shoveler/California/HKWF115/2007) HA H7N7 (A/Netherlands/219/03) HA H8N4 (A/pintail duck/Alberta/114/1979) HA H9N2 (A/Hong Kong/1073/99) HA H10N3 (A/duck/Hong Kong/786/1979) HA H11N2 (A/duck/Yangzhou/906/2002) HA H12N5 (A/green-winged teal/ALB/199/1991) HA H13N8 (A/black-headed gull/Netherlands/1/00) HA H15N8 (A/duck/AUS/341/1983) HA H16N3 (A/black-headed gull/Sweden/5/99) HA |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western Blot: Using a DAB detection system, the detection limit for Influenza B Hemagglutinin is approximately 12.5 ng/lane under non-reducing conditions and reducing conditions. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: Mouse Monoclonal Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569] - Analysis using HRP conjugated version of Mouse Monoclonal Influenza B Hemagglutinin Antibody (NBP3-06569H). The absorbance signal of antibody increases together with the weight of Influenza B (B/PHUKET/3073/2013) HA strain between 0.375 - 0.01 µg of HA range. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-06569_mouse-monoclonal-influenza-b-hemagglutinin-antibody-10h6c8g4-elisa-3102023133522..jpg "ELISA: Mouse Monoclonal Influenza B Hemagglutinin Antibody (10H6C8G4) [NBP3-06569] - Analysis using HRP conjugated version of Mouse Monoclonal Influenza B Hemagglutinin Antibody (NBP3-06569H). The absorbance signal of antibody increases together with the weight of Influenza B (B/PHUKET/3073/2013) HA strain between 0.375 - 0.01 µg of HA range. Image from verified customer review.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")