Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

Western Blot: Influenza A H1N1 PA Antibody - (A/WSN/1933) [NBP2-42874] - Analysis of 60 ug whole cell lysate. A: DF-1(WSN infected cell lysates) B: DF-1 cell lysates diluted at 1:250.

Non-infected (-) and infected (+) H1299 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PA protein antibody (NBP2-42874) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Influenza A virus PA protein antibody detects Influenza A virus PA protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Influenza A virus PA protein stained by Influenza A virus PA protein antibody (NBP2-42874) diluted at 1:500.Blue: Fluoroshield with DAPI .

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: V-Vi
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus PA (A/WSN/1933(H1N1)). The exact sequence is proprietary.
• Specificity: Influenza A H1N1 PA (A/WSN/1933)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:100-1:1000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Influenza A Virus H1N1.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Influenza A H1N1 PA Antibody - (A/WSN/1933) - BSA Free

• Influenza PA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Influenza A H1N1 PA Antibody (NBP2-42874)(1)

Publication using NBP2-42874

• Zhang H, Li H, Wang W et al. A unique feature of swine ANP32A provides susceptibility to avian influenza virus infection in pigs PLoS Pathog. 2020-02-21 [PMID: 32084248] (WB, Porcine)