Influenza A Virus M2 Antibody

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Western Blot: Influenza A Virus M2 Antibody [NBP2-14997] - Non-infected (-) and infected (+) H1299 whole cell extracts (15 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 ...read more
Immunocytochemistry/ Immunofluorescence: Influenza A Virus M2 Antibody [NBP2-14997] - Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: M2 protein stained by Influenza A ...read more
Western Blot: Influenza A Virus M2 Antibody [NBP2-14997] - Sample (50 ug of whole cell lysate) A: mock DF-1 cell lysates B: WSN-infected DF-1 cell lysates 15% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Influenza A Virus M2 Antibody Summary

Immunogen
Recombinant fragment corresponding to Internal region of Influenza A virus M2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Influenza A virus.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Background

Influenza A virus matrix 1, M1, is a critical protein required for assembly and budding. Hemagglutinin associates with M1 via its cytoplasmic tail and transmembrane domain. The M2 and nucleoprotein proteins are critical in the replication cycle of influenza viruses. The M2 channel protein is an essential component of the viral envelope because of its ability to form a highly selective, pH-regulated, proton-conducting channel. The M2 channel allows protons to enter the interior of the virus and acidification weakens the interaction of the M1 protein with the ribonuclear core.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

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