Influenza A Virus Antibody

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Western Blot: Influenza A Virus Antibody [NBP1-30320] - For the validation Influenza A Virus antibody in WB Assay, 3 ug of intact A/California/14/2009 H1N1 virions were loaded on SDS-PAGE, transferred to a PVDF membrane ...read more

Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Influenza A Virus Antibody Summary

Immunogen
A/California/14/2009 (H1N1) virions were used as an immunogen.
Specificity
This antibody detects the following H1N1 proteins: Hemagglutinin (HA) ~75 kDa, Neuraminidase monomer (NA) ~55 kDa, Matrix (M) ~26 kDa and Non-structural Protein monomer (NS) ~26-27 kDa. Reacts with A/New Caledonia/20/99, A/Brisbane/10/2007, A/California/14/2009 and likely reacts with most other isolates.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
Western Blot detection.

Reactivity Notes

Influenza A H1N1

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Influenza A Virus Antibody

  • Flu

Background

Influenzavirus A is a genus of the Orthomyxoviridae family of viruses and is a negative sense, single-stranded, segmented RNA virus. Based on the antigenicity of the glycoproteins, influenza A viruses are subdivided into sixteen H (H1-H16) and nine N (N1-N9) subtypes. A standard nomenclature for influenza virus isolates lists the influenza virus type (A or B), the host species (omitted if human origin), the geographical site, year of isolation, and the HA and NA subtype, for example: A/California/14/2009 (H1N1). The main antigenic determinants of influenza A and B viruses are the hemagglutinin (HA) and neuraminidase (NA) transmembrane glycoproteins. Projections of HA and NA cover the surface of the virus particle. NA forms a tetramer with an average molecular weight of 220 kDa (~55 kDa per monomer). The matrix (M) protein of influenza A virus is one of the two group-specific internal proteins of the virion, The non-structural protein (NP) exists as a homeodimer (molecular weight of 52 kDa) consisting of two identical monomers (each ~26 kDa).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Influenza A Virus Products

Influenza A Virus NBP1-30320

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