Influenza A H1N1 PB2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 PB2 Antibody [NBP2-42879] - Analysis of A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: PB2 protein stained by Influenza A H1N1 PB2 antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37 degrees C,30 min.

Western Blot: Influenza A H1N1 PB2 Antibody [NBP2-42879] - Analysis of 5 ug whole cell lysate. A: DF1 B: WSN virus infected DF1 10hr 7.5% SDS PAGE diluted at 1:5000.

Western Blot: Influenza A H1N1 PB2 Antibody [NBP2-42879] - 0.5 ug Influenza A viral lysate (0.5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 PB2 antibody (NBP2-42879) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-infected (-) and infected (+) H1299 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB2 protein antibody (NBP2-42879) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: V-Vi
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Influenza A H1N1 PB2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Influenza A H1N1 PB2 Antibody - BSA Free (NBP2-42879) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-Influenza A H1N1 PB2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of Influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation Assay dependent
  • Western Blot 1:1000-1:10000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Influenza A virus, Influenza A virus ( H1N1)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Influenza A H1N1 PB2 Antibody - BSA Free

• Influenza PB2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Influenza A H1N1 PB2 Antibody (NBP2-42879)(2)

Publications using NBP2-42879

• Zhang H, Li H, Wang W et al. A unique feature of swine ANP32A provides susceptibility to avian influenza virus infection in pigs PLoS Pathog. 2020-02-21 [PMID: 32084248] (WB, Porcine)
• Fatima U, Zhang Z, Zhang H Equine Mx1 Restricts Influenza A Virus Replication by Targeting at Distinct Site of its Nucleoprotein Viruses 2019-12-02 [PMID: 31810278] (WB, IP, Human, Canine)

FAQs for Influenza A H1N1 PB2 Antibody (NBP2-42879). (Showing 1 - 1 of 1 FAQs).

1. i want to know the difference about these two produce (NBP2-42878 and NBP2-42879). only protein content?
   • The main difference is the immunogen used to make them. The N-terminal region of Influenza A virus PB2 was used for NBP2-42878, and the C-terminus was used for NBP2-42879. Therefore, NBP2-42878 recognizes the N-terminal region, and NBP2-42879 recognizes the C-terminal region.