Influenza A H1N1 PB1 Antibody

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 PB1 Antibody [NBP2-42877] - Analysis of A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: PB1 protein stained by Influenza A H1N1 PB1 antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37-C,30 min.

Western Blot: Influenza A H1N1 PB1 Antibody [NBP2-42877] - Analysis of : A. 5 ug DF1 whole cell lysate/extract (untreated) B. 5 ug DF1 whole cell lysate/extract (WSN virus treatment for 10hr) 7.5 % SDS-PAGE Influenza A H1N1 PB1 antibody.

Non-infected (-) and infected (+) H1299 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza A virus PB1 protein antibody (NBP2-42877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

ANP32A proteins from different species interact with different polymerase trimeric complexes.DKO cells were transfected with different ANP32A (0.6μg) and polymerase plasmids (0.6μg PA, 1μg PB1, and 1μg PB2) from avian influenza viruses H7N9ZJ13(A), H9N2ZJ12(B), human influenza virus polymerase WSN (C). The cells were lysed at 24 h post-transfection. Co-IP was performed using Anti-FLAG M2 Magnetic Beads, followed by Western blotting to detect the ANP32A and viral proteins by using specific antibodies: PA antibody (NBP2-42874, NOVUS), PB1 antibody (NBP2-42877, NOVUS), PB2 antibody (NBP2-42879, NOVUS), Anti-Flag antibody (F1804, SIGMA). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32084248), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: V-Vi
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Influenza A H1N1 PB1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Influenza A virus

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Influenza A H1N1 PB1 Antibody

• Influenza PB1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Influenza A H1N1 PB1 Antibody (NBP2-42877)(2)

Publications using NBP2-42877

• Peacock TP, Sheppard CM, Lister MG et al. Mammalian ANP32A and ANP32B Proteins Drive Differential Polymerase Adaptations in Avian Influenza Virus Journal of virology 2023-05-31 [PMID: 37074204]
• Zhang H, Li H, Wang W et al. A unique feature of swine ANP32A provides susceptibility to avian influenza virus infection in pigs PLoS Pathog. 2020-02-21 [PMID: 32084248] (WB, Porcine)