Influenza A H1N1 Neuraminidase Antibody - (A/WSN/1933)

Western Blot: Influenza A H1N1 Neuraminidase Antibody - (A/WSN/1933) [NBP2-42870] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 NA (Neuraminidase) antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 Neuraminidase Antibody - (A/WSN/1933) [NBP2-42870] - Influenza A and B Virus infected cells slide. Green: Influenza A virus Nucleoprotein antibody diluted at 1:100.

Western Blot: Influenza A H1N1 Neuraminidase Antibody - (A/WSN/1933) [NBP2-42870] - Non-infected (-) and infected (+) H1299 whole cell extracts (5 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus H1N1 NA (Neuraminidase) antibody diluted at 1:1000.

Western Blot: Influenza A H1N1 Neuraminidase Antibody - (A/WSN/1933) [NBP2-42870] - Analysis of A. 5 ug DF-1 whole cell lysate/extract B. 5 ug whole cell lysate/extract WSN virus infected DF-1 cells 10 % SDS-PAGE Influenza A H1N1 Neuraminidase (NA) antibody dilution: 1:1000.

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 Neuraminidase Antibody - (A/WSN/1933) [NBP2-42870] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza A virus H1N1 NA (Neuraminidase) stained by Influenza A virus H1N1 NA (Neuraminidase) antibody diluted at 1:500.Blue: Fluoroshield with DAPI.

Western Blot: Influenza A H1N1 Neuraminidase Antibody - (A/WSN/1933) [NBP2-42870] - Influenza A viral lysates were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 Neuraminidase antibody (NBP2-42870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: V
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Influenza A H1N1 Neuraminidase Antibody - (A/WSN/1933) Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of Influenza A virus H1N1 NA (Neuraminidase) (A/WSN/1933(H1N1). The exact sequence is proprietary.
• Specificity: Influenza A H1N1 Neuraminidase (A/WSN/1933)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000
• Application Notes: ICC/IF-Assay dependent
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.