Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) [NBP2-14997] - Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: M2 protein stained by Influenza A H1N1 M2 antibody diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37CC, 30 min.

Western Blot: Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) [NBP2-14997] - Sample (50 ug of whole cell lysate) A: mock DF-1 cell lysates B: WSN-infected DF-1 cell lysates 15% SDS PAGE; antibody diluted at 1:5000.

Non-infected (-) and infected (+) H1299 whole cell extracts (15 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody (NBP2-14997) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: V-Vi
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free Summary

• Immunogen: Recombinant protein corresponding to Influenza A H1N1 M2 (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.
• Specificity: Influenza A H1N1 M2 (A/Puerto Rico/8/1934)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Influenza A H1N1 M2 Antibody - (A/Puerto Rico/8/1934) - BSA Free

• Influenza M2

Background

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Uniprot:
  • H1N1(Human)