Influenza A H1N1 M2 Antibody (HL1856) - Azide and BSA Free

Western Blot: Influenza A H1N1 M2 Antibody (HL1856) - Azide and BSA Free [NBP3-25525] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody [HL1856] (NBP3-25525) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Influenza A H1N1 M2 Antibody (HL1856) - Azide and BSA Free [NBP3-25525] - Non-infected (-) and infected (+) H1299 whole cell extract (15 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Influenza A virus M2 (matrix protein) antibody [HL1856] (NBP3-25525) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M2 Antibody (HL1856) - Azide and BSA Free [NBP3-25525] - Influenza A virus M2 (matrix protein) antibody [HL1856] detects Influenza A virus M2 (matrix protein) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza A virus M2 (matrix protein) stained by Influenza A virus M2 (matrix protein) antibody [HL1856] (NBP3-25525) diluted at 1:500. Blue: Fluoroshield with DAPI .

• Reactivity: V-Vi
• Applications: WB, ICC/IF
• Clone: HL1856
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Influenza A H1N1 M2 Antibody (HL1856) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of Influenza A H1N1 M2
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Influenza A H1N1 M2 Antibody (HL1856) - Azide and BSA Free

• Influenza M2

Background

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.