Influenza A H1N1 M1 Antibody - (A/Puerto Rico/8/1934) - BSA Free

Western Blot: Influenza A H1N1 M1 Antibody - (A/Puerto Rico/8/1934) [NBP2-14995] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A M1 (matrix protein) antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Influenza A H1N1 M1 Antibody - (A/Puerto Rico/8/1934) [NBP2-14995] - Sample (50 ug of whole cell lysate) A: mock DF-1 cell lysates B: WSN-infected DF-1 cell lysates 15% SDS PAGE; antibody diluted at 1:5000.

• Reactivity: V-Vi
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Influenza A H1N1 M1 Antibody - (A/Puerto Rico/8/1934) - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of Influenza A H1N1 M1 (A/Puerto Rico/8/1934(H1N1). The exact sequence is proprietary.
• Specificity: Influenza A H1N1 M1. (A/Puerto Rico/8/1934)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000-1:10000
• Application Notes: ICC/IF- Assay-dependent dilution
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Background

Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The matrix protein of influenza virus is a multimer of 25-30 kDa subunits that mediates the encapsidation of the RNA-nucleoprotein cores into the memrane envelope (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Influenza A H1N1 M1 Antibody (NBP2-14995)(1)

Publication using NBP2-14995

• Si L, Shen Q, Li J et al. Generation of a live attenuated influenza A vaccine by proteolysis targeting Nature biotechnology 2022-07-04 [PMID: 35788567] (WB, ICC/IF, Influenza A Virus H1N1)
  
  Details:
  1:1,000 dilution for western blotting, 1:200 dilution for immunofluorescence staining