![Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free [NBP3-25524] - Immunofluorescent analysis of Influenza virus infected cells using Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524). Sample: Influenza A and B virus infected cells slide. Green: Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25524_rabbit-influenza-a-h1n1-m1-mab-hl1273-azide-and-bsa-free-2120249342752.jpg "Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free [NBP3-25524] - Immunofluorescent analysis of Influenza virus infected cells using Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524). Sample: Influenza A and B virus infected cells slide. Green: Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524) diluted at 1:100. Blue: Fluoroshield with DAPI .")
| Reactivity | V-ViSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | HL1273 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free (NBP3-25524) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment corresponding to C-terminal region of Influenza A virus M1 (A/Puerto Rico/8/1934(H1N1)). The exact sequence is proprietary. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free [NBP3-25524] - Non-infected (-) and infected (+) H1299 whole cell extracts (15 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25524_rabbit-influenza-a-h1n1-m1-mab-hl1273-azide-and-bsa-free-2120249362731.jpg "Western Blot: Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free [NBP3-25524] - Non-infected (-) and infected (+) H1299 whole cell extracts (15 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free [NBP3-25524] - Influenza A virus M1 (matrix protein) antibody [HL1273] detects Influenza A virus M1 (matrix protein) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded Influenza A virus M1 transfected 293T. Green: Influenza A virus M1 (matrix protein) stained by Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25524_rabbit-influenza-a-h1n1-m1-mab-hl1273-azide-and-bsa-free-2120249362780.jpg "Immunohistochemistry-Paraffin: Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free [NBP3-25524] - Influenza A virus M1 (matrix protein) antibody [HL1273] detects Influenza A virus M1 (matrix protein) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded Influenza A virus M1 transfected 293T. Green: Influenza A virus M1 (matrix protein) stained by Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free [NBP3-25524] - Influenza A virus M1 (matrix protein) antibody [HL1273] detects Influenza A virus M1 (matrix protein) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza A virus M1 (matrix protein) stained by Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25524_rabbit-influenza-a-h1n1-m1-mab-hl1273-azide-and-bsa-free-2120249415948.jpg "Immunocytochemistry/Immunofluorescence: Influenza A H1N1 M1 Antibody (HL1273) - Azide and BSA Free [NBP3-25524] - Influenza A virus M1 (matrix protein) antibody [HL1273] detects Influenza A virus M1 (matrix protein) protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Influenza A virus M1 (matrix protein) stained by Influenza A virus M1 (matrix protein) antibody [HL1273] (NBP3-25524) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 M1 antibody [HL1273] (NBP3-25524) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25524_rabbit-influenza-a-h1n1-m1-mab-hl1273-azide-and-bsa-free-western-blot-1411202518273418.jpg "Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A H1N1 M1 antibody [HL1273] (NBP3-25524) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")