Influenza A H1N1 Hemagglutinin Antibody (2F1A7) - Azide and BSA Free

Western Blot: Influenza A H1N1 Hemagglutinin Antibody (2F1A7) [NBP3-06443] - Anti- Influenza A H1N1 Hemagglutininmouse monoclonal antibody at 1:1000 dilution.Sample: Reocombinant Protein 10 ngLane A: H3N2 (A/Darwin/9/2021) HA ProteinLane B: H3N2 (A/Darwin/9/2021) HA ProteinLane C: H3N2 (A/Darwin/6/2021) HA ProteinSecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.

• Reactivity: V
• Applications: WB, ELISA, ELISA
• Clone: 2F1A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Influenza A H1N1 Hemagglutinin Antibody (2F1A7) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A H1N1 (Accession#: ABD77675.1; Met 1-Gln 528).
• Specificity: No cross-reactivity in ELISA with:
  
  H1N1 (A/BrevigMission/1/1918) HA
  H1N1 (A/Brisbane/02/2018) HA
  H1N1 (A/Brisbane/59/2007) HA
  H1N1 (A/Brisbane/59/2007) HA1
  H1N1 (A/California/04/2009) HA
  H1N1 (A/California/04/2009) HA1
  H1N1 (A/California/07/2009) HA
  H1N1 (A/Guangdong-Maonan/SWL1536/2019) / (A/Hawaii/70/2019) HA
  H1N1 (A/Michigan/45/2015) HA
  H1N1 (A/New Caledonia/20/1999) HA
  H1N1 (A/Ohio/UR06-0091/2007) HA
  H1N1 (A/Solomon Islands/3/2006)
  H1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HA
  H1N1 (A/WSN/1933) HA
  H1N2 (A/swine/Guangxi/13/2006) HA
  H1N3 (A/duck/NZL/160/1976)
  H3N2 (A/Brisbane/10/2007) HA
  H3N2 (A/Cambodia/e0826360/2020) HA
  H3N2 (A/Hong Kong/2671/2019) HA
  H3N2 (A/Hong Kong/45/2019) HA
  H3N2 (A/Hong Kong/4801/2014) HA
  H3N2 (A/Kansas/14/2017) HA
  H3N2 (A/Singapore/INFIMH-16-0019/2016) HA
  H3N2 (A/Switzerland/9715293/2013) HA
  H5N1 (A/Anhui/1/2005) HA
  H5N1 (A/Anhui/1/2005) HA2
  Influenza B (B/Brisbane/60/2008) HA
  Influenza B (B/Colorado/06/2017) HA
  Influenza B (B/Florida/4/2006) HA
  Influenza B (B/PHUKET/3073/2013) HA
  Influenza B (B/Washington/02/2019) HA
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:2000
  • Sandwich ELISA Detection 1:1000-1:10000
  • Western Blot 1:500-1:1000
• Application Notes: Western Blot: Using a DAB detection system, the detection limit for Influenza A H1N1 (A/Puerto Rico/8/1934) hemagglutinin is approximately 8 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended. This antibody will detect Influenza A H1N1 Hemagglutinin (A/Puerto Rico/8/34) in ELISA pair set (NBP2-79358). In a sandwich ELISA, it can be used as a capture antibody when paired with (NBP3-30994).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.