| Reactivity | All-NASpecies Glossary |
| Applications | ELISA, AP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Store at 4C or -20 C. The working antibody solution is stable for at least 7 days at 4C. Precautions should be taken for storage for longer periods. Problems of long-term stability may occur with highly diluted solutions.
For long storage purposes in solution the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use. |
| Immunogen | BSA-Indole-3-butyric acid conjugate |
| Specificity | Target: Indole-3-butyric acid (IBA), CAS no.: 133-32-4, Solubility target: Methanol, ethanol. Cross Reactivity with Indole-3-actic acid: < 0.1 % |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralization of the extract.
Suggested dilution !:50,000 in the indirect ELISA and 1:12,800 in the direct ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/ml ovalbumin-conjugated indole-3-butyric acid. HRP-conjugated anti-rabbit IgG should be used as a tracer in a 1:8,000 dilution. Immuno-affinity-chromatography. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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