IgT Antibody (Z55F8*C3) - BSA Free

Western Blot: IgT Antibody (Z55F8*C3) - Azide and BSA Free [NBP2-61127] - Western blot showing detection of IgT in sera of gilt-head (sea) bream (Sparus aurata) Lumpsucker (Cyclopteridae) and Large yellow croaker (Larimichthys crocea) using Anti-IgT [Z55F8*C3].

Western Blot: IgT Antibody (Z55F8*C3) - Azide and BSA Free [NBP2-61127] - Western blot showing detection of IgT in meagre (Argyrosomus regius) serum using Anti-IgT [Z55F8*C3].

• Reactivity: Fi
• Applications: WB, ELISA, IHC
• Clone: Z55F8*C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IgT Antibody (Z55F8*C3) - BSA Free Summary

• Description: The clone number is listed as Z55F8*C3 in the scientific literature.
• Immunogen: Ovalbumin-conjugated synthetic peptide
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA neat
  • Immunohistochemistry 1:5
  • Western Blot neat
• Application Notes: Antigen retrieval for IHC: microwave 20 min @ 800W in 10 mM citrate buffer, pH 6.0. Positive control(s): ELISA - fish sera, peptide immunogen; Western Blot - Sera; IHC - Formalin-fixed, paraffin-embedded head kidney, gill and spleen tissues

Reactivity Notes

Gilthead seabream, Meagre and Lumpsucker

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IgT Antibody (Z55F8*C3) - BSA Free

• IgZ
• Immunoglobulin IgT

Background

Detection of specific antibodies in the serum of animals is an indicator of previous exposure to pathogens which is very valuable for broodstock health testing. This Anti-IgT antibody provides a useful tool to monitor vaccine performance in fish and will assist in the development of future vaccines, especially mucosal vaccines. Target species: Meagre (Argyrosomus regius), gilt-head (sea) bream (Sparus aurata), Lumpsucker (Cyclopteridae), Large yellow croaker (Larimichthys crocea).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.