![Immunohistochemistry-Paraffin: IGHG1 Antibody (IG1707R) [NBP2-53149] - Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Rabbit Recombinant Monoclonal Antibody (IG1707R).](http://images.novusbio.com/fullsize2/IGHG1-Antibody-(IG1707R)-Immunohistochemistry-Paraffin-NBP2-53149-img0001.jpg "Immunohistochemistry-Paraffin: IGHG1 Antibody (IG1707R) [NBP2-53149] - Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Rabbit Recombinant Monoclonal Antibody (IG1707R).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IHC-P, PAGE |
| Clone | IG1707R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Purified human Ig Gamma Chain |
| Localization | Cytoplasm, Cell Surface and Secreted |
| Marker | B-Cell Marker |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | IGHG1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | IHC-P:Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes |
| Theoretical MW | 75 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54449AF350 | |
| Alexa Fluor 405 | NBP2-54449AF405 | |
| Alexa Fluor 488 | NBP2-54449AF488 | |
| Alexa Fluor 532 | NBP2-54449AF532 | |
| Alexa Fluor 594 | NBP2-54449AF594 | |
| Alexa Fluor 647 | NBP2-54449AF647 | |
| Alexa Fluor 700 | NBP2-54449AF700 | |
| Alexa Fluor 750 | NBP2-54449AF750 | |
| Allophycocyanin | NBP2-54449APC | |
| Allophycocyanin/Cy7 | NBP2-54449APCCY7 | |
| Biotin | NBP2-54449B | |
| DyLight 350 | NBP2-54449UV | |
| DyLight 405 | NBP2-54449V | |
| DyLight 488 | NBP2-54449G | |
| DyLight 550 | NBP2-54449R | |
| DyLight 594 | NBP2-54449DL594 | |
| DyLight 650 | NBP2-54449C | |
| DyLight 680 | NBP2-54449FR | |
| DyLight 755 | NBP2-54449IR | |
| FITC | NBP2-54449F | |
| HRP | NBP2-54449H | |
| Janelia Fluor 549 | NBP2-54449JF549 | |
| Janelia Fluor 646 | NBP2-54449JF646 | |
| PE | NBP2-54449PE | |
| PE/Atto594 | NBP2-54449PEATT594 | |
| PE/Cy5.5 | NBP2-54449PECY55 | |
| PE/Cy7 | NBP2-54449PECY7 | |
| PerCP | NBP2-54449PCP |
Diseases for IGHG1 Antibody (NBP2-53149)Discover more about diseases related to IGHG1 Antibody (NBP2-53149).
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PTMs for IGHG1 Antibody (NBP2-53149)Learn more about PTMs related to IGHG1 Antibody (NBP2-53149).
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| Gene Symbol | IGHG1 |
![SDS-Page: IGHG1 Antibody (IG1707R) [NBP2-53149] - Analysis Purified IgG Rabbit Recombinant Monoclonal Antibody (IG1707R). Confirmation of Purity and Integrity of Antibody.](http://images.novusbio.com/fullsize2/IGHG1-Antibody-(IG1707R)-SDS-Page-NBP2-53149-img0002.jpg "SDS-Page: IGHG1 Antibody (IG1707R) [NBP2-53149] - Analysis Purified IgG Rabbit Recombinant Monoclonal Antibody (IG1707R). Confirmation of Purity and Integrity of Antibody.")
![Western Blot: RAG1 Antibody [NBP1-74190] - Mouse Liver Lysate 1ug/ml Gel Concentration 6-18%](http://images.novusbio.com/fullsize/RAG1 Antibody-Western Blot-NBP1-74190-img0001.jpg)
![Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Flow-(Intracellular)-NB200-540-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunocytochemistry-Immunofluorescence-NB200-540-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.](http://images.novusbio.com/fullsize2/Complement-C3-Antibody-(11H9)-Immunohistochemistry-Paraffin-NB200-540-img0004.jpg)
![Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/AICDA Antibody-Western Blot-NBP1-30955-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/AICDA Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30955-img0002.jpg)
![Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize2/AICDA-Antibody-Immunohistochemistry-Paraffin-NBP1-30955-img0005.jpg)
![Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/SPANXB1 Antibody-Western Blot-H00728695-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize2/SPANXB1-Antibody-Immunocytochemistry-Immunofluorescence-H00728695-B01P-img0003.jpg)
![Western Blot: BBS9 Antibody [NBP1-88927] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize2/BBS9-Antibody-Western-Blot-NBP1-88927-img0004.jpg)
![Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize2/BBS9-Antibody-Immunohistochemistry-Paraffin-NBP1-88927-img0002.jpg)
![Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Controls run before the experimental samples are analyzed, to adjust the fluorescent compensation on the flow cytometer due to the FITC signal from the Annexin V-FITC bleeding into the Propidium Iodide signal.](http://images.novusbio.com/fullsize2/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0003.jpg)
![Flow Cytometry: Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Adherent RAW cells treated with actinomycin-D, detached with a cell detachment solution and stained with the Annexin V Apoptosis Kit [FITC].](http://images.novusbio.com/fullsize2/Annexin-V-Apoptosis-Kit-[FITC]-Flow-Cytometry-NBP2-29373-img0001.jpg)
![Western Blot: RAG2 Antibody [NBP2-43666] - Analysis of A. 50 ug rat thymus lysate/extract 7.5 % SDS-PAGE RAG2 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize2/RAG2-Antibody-Western-Blot-NBP2-43666-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: RAG2 Antibody [NBP2-43666] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAG2 protein stained by RAG2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10um.](http://images.novusbio.com/fullsize2/RAG2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-43666-img0005.jpg)
![Immunohistochemistry-Paraffin: RAG2 Antibody [NBP2-43666] - Analysis of DLD1 Xenograft, using RAG2 antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/RAG2-Antibody-Immunohistochemistry-Paraffin-NBP2-43666-img0002.jpg)
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-87404-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/Adenosine-Deaminase-ADA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87404-img0013.jpg)
![Immunohistochemistry-Paraffin: Adenosine Deaminase/ADA Antibody [NBP1-87404] - Staining of human colon, duodenum, lymph node and testis using Anti-ADA antibody NBP1-87404 (A) shows similar protein distribution across tissues to independent antibody NBP1-90361 (B).](http://images.novusbio.com/fullsize2/Adenosine-Deaminase-ADA-Antibody-Immunohistochemistry-Paraffin-NBP1-87404-img0023.jpg)
![Immunohistochemistry-Paraffin: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - IgA2 stained human tonsil FFPE, 20X magnification. Antigen retrieval was performed with citrate pH 6 buffer. Primary antibody at 1:8000. Image from verified customer reivew.](http://images.novusbio.com/fullsize2/IgA2-Antibody-(RM125)-[Unconjugated]-Immunohistochemistry-Paraffin-NBP2-62025-img0005.jpg)
![Immunohistochemistry: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - Immunohistochemistry of Human Tonsil Tissue using NBP2-62025.](http://images.novusbio.com/fullsize2/IgA2-Antibody-(RM125)-[Unconjugated]-Immunohistochemistry-NBP2-62025-img0004.jpg)
![ELISA: IgA2 Antibody (RM125) [Unconjugated] [NBP2-62025] - ELISA of human immunoglobulins shows NBP2-62025 only reacts to human IgA2. No cross reactivity with human IgA1, IgG, IgM, IgD, or IgE. The plate was coated with different immunoglobulins.NBP2-62025 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/IgA2-Antibody-(RM125)-[Unconjugated]-ELISA-NBP2-62025-img0006.jpg)
![Western Blot: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Analysis of Haptoglobin on different cells lysates using anti-Haptoglobin antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: HepG2](http://images.novusbio.com/fullsize2/Haptoglobin Antibody (JM10-79)-Western Blot-NBP2-66881-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Staining Haptoglobin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Haptoglobin-Antibody-(JM10-79)-Immunocytochemistry-Immunofluorescence-NBP2-66881-img0003.jpg)
![Immunohistochemistry-Paraffin: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Analysis of paraffin-embedded mouse lung tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Haptoglobin-Antibody-(JM10-79)-Immunohistochemistry-Paraffin-NBP2-66881-img0007.jpg)
![Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Western-Blot-NBP1-43435-img0002.jpg)
![Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. Quadrant lines represent Rat IgG2a isotype control staining levels and cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize2/MS4A1-CD20-Antibody-(AISB12)-Flow-Cytometry-NBP1-43435-img0004.jpg)
![Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/PGK1-Antibody-Western-Blot-NBP2-19784-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody diluted at 1:20. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/PGK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19784-img0014.jpg)
![Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/PGK1-Antibody-Immunohistochemistry-Paraffin-NBP2-19784-img0008.jpg)
