IgG2c Antibody (RM223)

Western Blot: IgG2c Antibody (RM223) [Unconjugated] [NBP2-62013] - Western blot of nonreduced(-) and reduced(+) Mouse IgG2c, using NBP2-62013. This antibody reacts to nonreduced IgG2c.

ELISA: IgG2c Antibody (RM223) [Unconjugated] [NBP2-62013] - ELISA of IgG from BalB/C and C57BL6 shows NBP2-62013 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a.NBP2-62013 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.

ELISA: IgG2c Antibody (RM223) [Unconjugated] [NBP2-62013] - A titer ELISA of Mouse IgG2c. The plate was coated with different amounts of Mouse IgG2c. A serial dilution of NBP2-62013 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clone: RM223
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IgG2c Antibody (RM223) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse IgG2c
• Specificity: This antibody reacts to the Fc region of mouse IgG2c.No cross reactivity with mouse IgG1, IgG2a, IgG2b,IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.01 ug/ml - 0.2 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 0.5 ug/ml-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS with 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.