![Western Blot: IgG2c Antibody (RM223) [Unconjugated] [NBP2-62013] - Western blot of nonreduced(-) and reduced(+) Mouse IgG2c, using NBP2-62013. This antibody reacts to nonreduced IgG2c.](http://images.novusbio.com/fullsize/IgG2c-Antibody-RM223-Unconjugated-Western-Blot-NBP2-62013-img0005.jpg "Western Blot: IgG2c Antibody (RM223) [Unconjugated] [NBP2-62013] - Western blot of nonreduced(-) and reduced(+) Mouse IgG2c, using NBP2-62013. This antibody reacts to nonreduced IgG2c.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | RM223 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit IgG2c Antibody (RM223) (NBP2-62013) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Mouse IgG2c |
| Specificity | This antibody reacts to the Fc region of mouse IgG2c.No cross reactivity with mouse IgG1, IgG2a, IgG2b,IgG3, IgM, IgA, IgE, human IgG, or rat IgG. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol/PBS, 1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013] - ELISA of IgG from BalB/C and C57BL6 shows RM223 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM223 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62013_rabbit-monoclonal-igg2c-antibody-rm223-elisa-189202523435624.png "ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013] - ELISA of IgG from BalB/C and C57BL6 shows RM223 reacts to C57BL6 IgG containing IgG2c, and does not react to BalB/C IgG containing IgG2a. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM223 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.")
![ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013] - A titer ELISA of Mouse IgG2c. The plate was coated with different amounts of Mouse IgG2c. A serial dilution of RM223 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.](http://images.novusbio.com/fullsize/nbp2-62013_rabbit-monoclonal-igg2c-antibody-rm223-elisa-18920252337114.png "ELISA: IgG2c Antibody (RM223) - Azide and BSA Free [NBP2-62013] - A titer ELISA of Mouse IgG2c. The plate was coated with different amounts of Mouse IgG2c. A serial dilution of RM223 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")