![Flow Cytometry: Rat IgG2B Isotype Control (LF2) [NBP2-21941] - A surface stain was performed on human peripheral blood lymphocytes with CD8 alpha (YTC128.20) antibody NB100-64922AF647 and a matched isotype control NBP2-21941AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-24867AF488.](http://images.novusbio.com/fullsize/Rat-IgG2B-Isotype-Control-LF2-Flow-Cytometry-NBP2-21941-img0001.jpg "Flow Cytometry: Rat IgG2B Isotype Control (LF2) [NBP2-21941] - A surface stain was performed on human peripheral blood lymphocytes with CD8 alpha (YTC128.20) antibody NB100-64922AF647 and a matched isotype control NBP2-21941AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-24867AF488.")
| Reactivity | RtSpecies Glossary |
| Clone | LF2 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Description | This Rat IgG2B (LF2) immunoglobulin is useful as an isotype control for Rat IgG2B (LF2) antibody. This antibody is purified from culture supernatant of a hybridoma (Mouse myeloma fused with spleen cells from a Rat, clone LF2) with protein A/G. Endotoxin level < 0.7 EU/mg. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Rat |
| Purity | Protein A or G purified |
| Application Notes | Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from a 0.2 um filtered solution in PBS. 0.5mg and 0.25 mg sizes are provided in liquid form, PBS |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Protein A or G purified |
| Reconstitution Instructions | Reconstitute with PBS. |
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