Rat IgG2b Isotype Control (141945) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Ctrl
• Clone: 141945
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

Rat IgG2b Isotype Control (141945) [Phycoerythrin] Summary

• Immunogen: KLH
• Specificity: Selected for use as a rat IgG_2B isotype control for flow cytometry applications.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Control 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IgG2b Isotype Control (141945) [Phycoerythrin]

• IgG2B

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2b Isotype Control (IC013P)(8)

Publications using IC013P

• M Felsenstei, A Blank, AD Bungert, A Mueller, A Ghori, I Kremenetsk, O Rung, T Broggini, K Turkowski, L Scherschin, J Raggatz, P Vajkoczy, S Brandenbur CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology Cancers (Basel), 2020-07-13;12(7):. 2020-07-13 [PMID: 32668709] (Flow Cytometry, Mouse)
• Xiaolong Huang, Jun Wan, Dewen Leng, Yingying Zhang, Shuo Yang Dual‑targeting nanomicelles with CD133 and CD44 aptamers for enhanced delivery of gefitinib to two populations of lung cancer‑initiating cells Experimental and Therapeutic Medicine 2019-11-19 [PMID: 31853290]
• S Sriram, G Shaginurov, JT Tossberg, C Natarajan, CF Spurlock, TM Aune Longitudinal changes in the expression of IL-33 and IL-33 regulated genes in relapsing remitting MS PLoS ONE, 2018-12-18;13(12):e0208755. 2018-12-18 [PMID: 30562364] (Flow Cytometry, Human)
• Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Flow Cytometry, Human)
• Kumase F, Takeuchi K, Morizane Y, Suzuki J, Matsumoto H, Kataoka K, Al-Moujahed A, Maidana D, Miller J, Vavvas D AMPK-Activated Protein Kinase Suppresses Ccr2 Expression by Inhibiting the NF-kappaB Pathway in RAW264.7 Macrophages. PLoS ONE, 2016-01-22;11(1):e0147279. 2016-01-22 [PMID: 26799633] (Flow Cytometry Control, Mouse)
• Zheng , Jiao, Yang , Min, Shao , Jianghua, Miao , Yanju, Han , Jiahuai, Du , Jie Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasis. Mol Cancer, 2013-11-18;12(1):141. 2013-11-18 [PMID: 24245985] (Flow Cytometry Control, Mouse)
• Michail S. Lionakis, Muthulekha Swamydas, Brett G. Fischer, Theo S. Plantinga, Melissa D. Johnson, Martin Jaeger et al. CX3CR1-dependent renal macrophage survival promotes Candida control and host survival Journal of Clinical Investigation 2013-12-02 [PMID: 24177428] (Flow Cytometry Control, Transgenic Mouse)
• Michail S. Lionakis, Brett G. Fischer, Jean K. Lim, Muthulekha Swamydas, Wuzhou Wan, Chyi-Chia Richard Lee et al. Chemokine Receptor Ccr1 Drives Neutrophil-Mediated Kidney Immunopathology and Mortality in Invasive Candidiasis PLoS Pathogens 2012-08-16 [PMID: 22916017]