Rat IgG2b Isotype Control (141945) [Allophycocyanin]

Mouse splenocytes treated with 1 µg/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB168A, filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Ctrl
• Clone: 141945
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

Rat IgG2b Isotype Control (141945) [Allophycocyanin] Summary

• Immunogen: KLH
• Specificity: Selected for use as a rat IgG_2B isotype control for flow cytometry applications.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Control 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IgG2b Isotype Control (141945) [Allophycocyanin]

• IgG2B

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2b Isotype Control (IC013A)(7)

Publications using IC013A

• Gao, H;Di, J;Clausen, BH;Wang, N;Zhu, X;Zhao, T;Chang, Y;Pang, M;Yang, Y;He, R;Wang, Y;Zhang, L;Liu, B;Qiu, W;Lambertsen, KL;Brambilla, R;Rong, L; Distinct myeloid population phenotypes dependent on TREM2 expression levels shape the pathology of traumatic versus demyelinating CNS disorders Cell reports 2023-06-07 [PMID: 37289590] (Flow Cytometry, Mouse)
• M Binnewies, JL Pollack, J Rudolph, S Dash, M Abushawish, T Lee, NS Jahchan, P Canaday, E Lu, M Norng, S Mankikar, VM Liu, X Du, A Chen, R Mehta, R Palmer, V Juric, L Liang, KP Baker, L Reyno, MF Krummel, M Streuli, V Sriram Targeting TREM2 on tumor-associated macrophages enhances immunotherapy Cell Reports, 2021-10-19;37(3):109844. 2021-10-19 [PMID: 34686340] (Flow Cytometry, Human)
• Mingjuan Wu, Jun Liu, Fanying Li, Shuoya Huang, Jingxin He, Yunxia Xue et al. Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution Mucosal Immunology 2020-01-01 [PMID: 31434991]
• SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors Cancer Res, 2017-02-15;0(0):. 2017-02-15 [PMID: 28202513] (Flow Cytometry, Mouse)
• Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells. J. Immunol., 2012-06-29;189(3):1162-72. 2012-06-29 [PMID: 22753933] (Control, Mouse)
• Targeted inhibition of T-cell factor activity promotes syndecan-2 expression and sensitization to doxorubicin in osteosarcoma cells and bone tumors in mice. J. Bone Miner. Res., 2012-10-01;27(10):2118-29. 2012-10-01 [PMID: 22550000] (Control, Human)
• Ahern D, Lloyd CM, Robinson DS Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors. Allergy, 2009-02-07;64(8):1121-9. 2009-02-07 [PMID: 19208087] (Flow Cytometry, Human)