Rat IgG2b Isotype Control (141945) [Fluorescein]

Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2160F, filled histogram) or Rat IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Ctrl
• Clone: 141945
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

Rat IgG2b Isotype Control (141945) [Fluorescein] Summary

• Immunogen: KLH
• Specificity: Selected for use as a rat IgG_2B isotype control for flow cytometry applications.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Control 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IgG2b Isotype Control (141945) [Fluorescein]

• IgG2B

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2b Isotype Control (IC013F)(5)

Publications using IC013F

• OJ Lara-Guzmá, Á Gil-Izquie, S Medina, E Osorio, R Álvarez-Qu, N Zuluaga, C Oger, JM Galano, T Durand, K Muñoz-Dura Oxidized LDL triggers changes in oxidative stress and inflammatory biomarkers in human macrophages Redox Biol, 2017-11-22;15(0):1-11. 2017-11-22 [PMID: 29195136] (Flow Cytometry, Human)
• Yamada T, Abei M, Danjoh I, Shirota R, Yamashita T, Hyodo I, Nakamura Y Identification of a unique hepatocellular carcinoma line, Li-7, with CD13(+) cancer stem cells hierarchy and population change upon its differentiation during culture and effects of sorafenib. BMC Cancer, 2015-04-11;15(0):260. 2015-04-11 [PMID: 25885470] (Flow Cytometry, Human)
• Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation. J Immunol, 2013-02-27;190(7):3696-705. 2013-02-27 [PMID: 23447686] (Flow Cytometry, Mouse)
• Jörg C. Gerlach, Marc Lübberstedt, Josefina Edsbagge, Alexander Ring, Mariah Hout, Matt Baun et al. Interwoven Four-Compartment Capillary Membrane Technology for Three-Dimensional Perfusion with Decentralized Mass Exchange to Scale Up Embryonic Stem Cell Culture Cells Tissues Organs 2010-01-01 [PMID: 20197653]
• Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G TREM-1 promotes survival during septic shock in mice. Eur. J. Immunol., 2007-02-01;37(2):456-66. 2007-02-01 [PMID: 17230441] (Control, Mouse)