![ELISA: Mouse IgG2a Isotype Control (M2A) [Biotin] [NBP1-96889] - ELISA Results of Mouse IgG2a isotype control (M2A) [Biotin]. Each well was coated in duplicate with 1.0 ug of Mouse IgG2a Isotype Control Biotin Conjugate. The working dilution is 364,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin-HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/Mouse-IgG2a-Isotype-Control-M2A-Biotin-ELISA-NBP1-96889-img0001.jpg "ELISA: Mouse IgG2a Isotype Control (M2A) [Biotin] [NBP1-96889] - ELISA Results of Mouse IgG2a isotype control (M2A) [Biotin]. Each well was coated in duplicate with 1.0 ug of Mouse IgG2a Isotype Control Biotin Conjugate. The working dilution is 364,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin-HRP conjugated and TMB substrate.")
| Reactivity | MuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF |
| Clone | M2A |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-96848 | |||
| HRP | NBP1-97075 | |||
| Texas Red | NBP1-96794 | |||
| Unconjugated | NBP1-96778 | |||
| Description | Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. This antibody has been prepared from in vitro cell culture by selective precipitation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2a was proven by Dot Blot. |
| Specificity | Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Mouse IgG2a was proven by Dot Blot. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Selective Precipitation |
| Dilutions |
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| Application Notes | This product has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. |
| Storage | Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Concentration | LYOPH |
| Purity | Selective Precipitation |
| Reconstitution Instructions | Reconstitute with 100 ul deionized water (or equivalent). |
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