Mouse IgG2a Isotype Control (M2A)

Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 cells treated for 3h with vehicle or perifosine (0.5, 1 or 2.5 uM) were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.

Immunoprecipitation: Mouse IgG2a Isotype Control (M2A) [NBP1-96778] - Cell lysates from T24 BC cells either untreated or treated with sorafenib (20 uM) for 1 or 3 h were immunoprecipitated (IP) using a monoclonal anti-CB Ab. The immunoprecipitates were separated via 12% SDS-PAGE and then immunoblotted (IB) using Abs against phospho-tyrosine (anti-pTyr) and CB. The lysates were also immunoprecipitated using isotype-matched IgG, which was used as a control. Image collected and cropped by CiteAb from the following publication (impactjournals.com/oncoscience/index.php?abs=147), licensed under a CC-BY license.

SDS-Page Results of Mouse IgG2a Isotype Control (M2A).Lane 1: Mouse IgG2a Isotype Control (M2A) Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight Marker

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, IP
• Clone: M2A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Purified

Mouse IgG2a Isotype Control (M2A) Summary

• Description: This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.
  
  Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Specificity: Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Mouse IgG2a Isotype Control (M2A)

• IgG2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (NBP1-96778)(4)

Publications using NBP1-96778

• Kawasaki Y, Suzuki H, Miura M et al. LAT1 is associated with poor prognosis and radioresistance in head and neck squamous cell carcinoma Oncology letters 2023-04-01 [PMID: 36970606] (Flow Cytometry)
• Cayli S, Sahin C, Sanci TO, Nakkas H Inhibition of p97/VCP function leads to defective autophagosome maturation, cell cycle arrest and apoptosis in mouse Sertoli cells Theriogenology 2020-09-15 [PMID: 32966945] (ICC/IF)
• Shin W, Jeon Y, Choi I, Kim YJ. V-set and Ig domain-containing 4 (VSIG4)-expressing hepatic F4/80+ cells regulate oral antigen-specific responses in mouse. Eur. J. Immunol. 2017-12-13 [PMID: 29235119] (Mouse)
  
  Details:
  This citation used the FITC form of this antbody
• Amantini C, Morelli MB, Santoni M et al. Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells. Oncoscience 2015-01-01 [PMID: 26097873]

FAQs for IgG2a Isotype Control (NBP1-96778). (Showing 1 - 2 of 2 FAQs).

1. I would like to know the clonality of this sample. Is this monoclonal or polyclonal?
   • Their is no clonality for this product as it is an isotype control, and contains multiple different non-specific IgG2a immunoglobulins that are non-specific, to rule out binding with Fc receptors in flow and other experiments requiring this type of control.
2. I want to know how the Ab was purified. More specifically, if it was with chomatography (like anti-mouse IgG columns, or other) OR with protein A or protein G columns. The latter is important to us to take presence of trace amounts of protein A or protein G into consideration.
   • This sample is a mouse IgG2a Isotype Control, which has been prepared from immunodeficient mouse ascites by protein A chromatography using specific conditions for subclass purification. Typically less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA.