Mouse IgG2a Isotype Control (M2A) [FITC]

Dot Blot: Mouse IgG2a Isotype Control (M2A) [FITC] [NBP1-96848] - Dot Blot of Mouse IgG2a isotype control (M2A) [FITC]. Ms IgG2a Isotype Control FITC Conjugate (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Block: Fluorescent Buffer RT for 30 mins.

• Reactivity: Mu
• Applications: WB, DB, ELISA, Flow, ICC/IF
• Clone: M2A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC
• Concentration: LYOPH

Mouse IgG2a Isotype Control (M2A) [FITC] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This antibody has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2a was proven by Dot Blot.
• Specificity: Extensive cross-adsorbtion was performed to remove any unwanted subclasses. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2a was proven by Dot Blot.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, Immunofluorescence, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent).

Alternate Names for Mouse IgG2a Isotype Control (M2A) [FITC]

• IgG2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (NBP1-96848)(2)

Publications using NBP1-96848

• Kawasaki Y, Suzuki H, Miura M et al. LAT1 is associated with poor prognosis and radioresistance in head and neck squamous cell carcinoma Oncology letters 2023-04-01 [PMID: 36970606] (Flow Cytometry)
• Shin W, Jeon Y, Choi I, Kim YJ. V-set and Ig domain-containing 4 (VSIG4)-expressing hepatic F4/80+ cells regulate oral antigen-specific responses in mouse. Eur. J. Immunol. 2017-12-13 [PMID: 29235119] (Mouse)
  
  Details:
  This citation used the FITC form of this antbody