Mouse IgG2a Isotype Control (20102) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: Ctrl
• Clone: 20102
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Mouse IgG2a Isotype Control (20102) [Alexa Fluor® 594] Summary

• Immunogen: KLH
• Specificity: Selected for use as mouse IgG_2A isotype control for flow cytometry applications.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Control 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IgG2a Isotype Control (20102) [Alexa Fluor® 594]

• IgG2A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG2a Isotype Control (IC003T)(4)

Publications using IC003T

• Licaj, M;Mhaidly, R;Kieffer, Y;Croizer, H;Bonneau, C;Meng, A;Djerroudi, L;Mujangi-Ebeka, K;Hocine, HR;Bourachot, B;Magagna, I;Leclere, R;Guyonnet, L;Bohec, M;Guérin, C;Baulande, S;Kamal, M;Le Tourneau, C;Lecuru, F;Becette, V;Rouzier, R;Vincent-Salomon, A;Gentric, G;Mechta-Grigoriou, F; Residual ANTXR1+ myofibroblasts after chemotherapy inhibit anti-tumor immunity via YAP1 signaling pathway Nature communications 2024-02-12 [PMID: 38346978] (Flow Cytometry Control, Human)
• Ilaria Magagna, Nicolas Gourdin, Yann Kieffer, Monika Licaj, Rana Mhaidly, Pascale Andre et al. CD73-Mediated Immunosuppression Is Linked to a Specific Fibroblast Population That Paves the Way for New Therapy in Breast Cancer Cancers (Basel) 2021-11-23 [PMID: 34884993] (Flow Cytometry Control)
• Y Kieffer, HR Hocine, G Gentric, F Pelon, C Bernard, B Bourachot, S Lameiras, L Albergante, C Bonneau, A Guyard, K Tarte, A Zinovyev, S Baulande, G Zalcman, A Vincent-Sa, F Mechta-Gri Single-cell analysis reveals fibroblast clusters linked to immunotherapy resistance in cancer Cancer Discov, 2020-05-20;0(0):. 2020-05-20 [PMID: 32434947] (Flow Cytometry, Human)
• F Pelon, B Bourachot, Y Kieffer, I Magagna, F Mermet-Mei, I Bonnet, A Costa, AM Givel, Y Attieh, J Barbazan, C Bonneau, L Fuhrmann, S Descroix, D Vignjevic, P Silberzan, MC Parrini, A Vincent-Sa, F Mechta-Gri Cancer-associated fibroblast heterogeneity in axillary lymph nodes drives metastases in breast cancer through complementary mechanisms Nat Commun, 2020-01-21;11(1):404. 2020-01-21 [PMID: 31964880] (Flow Cytometry, Human)